Tissue-resident memory T (T-RM) cells are characterized by their surface expression of CD69 and can be subdivided in CD103+ and CD103-T-RM cells. The origin and functional characteristics of T-RM... Show moreTissue-resident memory T (T-RM) cells are characterized by their surface expression of CD69 and can be subdivided in CD103+ and CD103-T-RM cells. The origin and functional characteristics of T-RM cells in the renal allograft are largely unknown. To determine these features we studied T-RM cells in transplant nephrectomies. T-RM cells with a CD103+ and CD103-phenotype were present in all samples (n = 13) and were mainly CD8+ T cells. Of note, donor-derived T-RM cells were only detectable in renal allografts that failed in the first month after transplantation. Grafts, which failed later, mainly contained recipient derived T-RM cells. The gene expression profiles of the recipient derived CD8+ T-RM cells were studied in more detail and showed a previously described signature of tissue residence within both CD103+ and CD103-T-RM cells. All CD8+ T-RM cells had strong effector abilities through the production of IFN gamma and TNF alpha, and harboured high levels of intracellular granzyme B and low levels of perforin. In conclusion, our results demonstrate that donor and recipient T-RM cells reside in the rejected renal allograft. Over time, the donor-derived T-RM cells are replaced by recipient T-RM cells which have features that enables these cells to aggressively respond to the allograft. Show less
Eikmans, M.; Canck, I. de; Pol, P. van der; Baan, C.C.; Haasnoot, G.W.; Mallat, M.J.K.; ... ; Claas, F.H.J. 2012
