Neutral loss (NL) spectral data presents a mirror of MS2 data and is a valuable yet largely untapped resource for molecular discovery and similarity analysis. Tandem mass spectrometry (MS2) data is... Show moreNeutral loss (NL) spectral data presents a mirror of MS2 data and is a valuable yet largely untapped resource for molecular discovery and similarity analysis. Tandem mass spectrometry (MS2) data is effective for the identification of known molecules and the putative identification of novel, previously uncharacterized molecules (unknowns). Yet, MS2 data alone is limited in characterizing structurally related molecules. To facilitate unknown identification and complement the METLIN-MS2 fragment ion database for characterizing structurally related molecules, we have created a MS2 to NL converter as a part of the METLIN platform. The converter has been used to transform METLIN's MS2 data into a neutral loss database (METLIN-NL) on over 860 000 individual molecular standards. The platform includes both the MS2 to NL converter and a graphical user interface enabling comparative analyses between MS2 and NL data. Examples of NL spectral data are shown with oxylipin analogues and two structurally related statin molecules to demonstrate NL spectra and their ability to help characterize structural similarity. Mirroring MS2 data to generate NL spectral data offers a unique dimension for chemical and metabolite structure characterization. Show less
Algaba, J.C.; Anczarski, J.; Asada, K.; Baloković, M.; Chandra, S.; Cui, Y.-Z.; ... ; Doroshenko, V. et al. 2021
Previous studies have suggested that female voices may impede verbal processing. For example, words were remembered less well and lexical decision was slower when spoken by a female speaker. The... Show morePrevious studies have suggested that female voices may impede verbal processing. For example, words were remembered less well and lexical decision was slower when spoken by a female speaker. The current study tried to replicate this gender effect in an auditory semantic/associative priming task that excluded any effects of speaker variability and extended previous research by examining the role of two voice features important in perceived gender: pitch and formant frequencies. Additionally, listener gender was included in the experimental design. Results show that, contrary to previous findings, there is no evidence that a lexical decision of a target word is slower when spoken by a female speaker than by a male speaker for female and male listeners. Additionally, the semantic/associative priming effect was not affected by speaker gender, neither did female mean pitch or formants predict the semantic/associative priming effect. At the behavioural level, the current study found no evidence for a gender effect in a semantic/associative priming task. Show less
