Comparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles-Part 1: Separation-based methods

Reusch, D.; Haberger, M.; Maier, B.; Maier, M.; Kloseck, R.; Zimmermann, B.; ... ; Wuhrer, M. (2015)

Article / Letter to editor

• IgG glycosylation
• monoclonal antibody (mAb)
• HILIC-UPLC
• 2-AB labeling
• APTS labeling
• method comparison
• DNA analyzer
• HPAEC
• glycan analysis
• CE-LIF
• high-throughput
• mAb
• monoclonal antibody
• Fc
• fragment crystallizable
• IgG
• immunoglobulin G
• hydrophilic interaction liquid chromatography-ultra high performance liquid chromatography
• 2-AB
• 2-aminobenzamide
• Fab
• fragment
• antigen-binding
• capillary electrophoresis-laser induced fluorescence
• HPLC
• high performance liquid chromatography
• MALDI-MS
• matrix-assisted laser desorption
• ionization-mass spectrometry
• ESI-MS
• electrospray ionization-mass spectrometry
• HPAEC-PAD
• high-performance anion exchange chromatography with pulsed amperometric detection
• APTS
• 8-aminopyrene-1
• 3
• 6-trisulfonic acid
• DSA-FACE
• DNA-sequencer-aided fluorophore-assisted carbohydrate electrophoresis
• ANTS
• 8-aminonaphthalene-1
• 6-trisulfonate
• CCGE
• cartridge-based capillary gel electrophoresis
• HR
• high resolution
• IAB
• InstantAB labeling
• CHO
• Chinese hamster ovary

• All authors: Reusch, D.; Haberger, M.; Maier, B.; Maier, M.; Kloseck, R.; Zimmermann, B.; Hook, M.; Szabo, Z.; Tep, S.; Wegstein, J.; Alt, N.; Bulau, P.; Wuhrer, M.
• Date: 2015-02-28
• Journal: mAbs
• Volume: 7
• Issue: 1
• Pages: 167 - 179
• DOI: doi:10.4161/19420862.2014.986000