MALDI-TOF mass spectrometry for differentiation between Streptococcus pneumoniae and Streptococcus pseudopneumoniae

Prehn, J. van; Veen, S.Q. van; Schelfaut, J.J.G.; Wessels, E. (2016)

Article / Letter to editor

We compared the Vitek MS and Microflex MALDI-TOF mass spectrometry platform for species differentiation within the Streptococcus miffs group with PCR assays targeted at lytA, Spn9802, and recA as reference standard. The Vitek MS correctly identified 10/11 Streptococcus pneumoniae, 13/13 Streptococcus pseudopneumoniae, and 12/13 S. mitis/oralis. The Microflex correctly identified 9/11 S. pneumoniae, 0/13 S. pseudopneumoniae, and 13/13 S. mitis/oralis. MALDI-TOF is a powerful tool for species determination within the mitis group. Diagnostic accuracy varies depending on platform and database used. (C) 2016 The Authors. Published by Elsevier Inc.

• Streptococcus
• Mitis group
• MALDI-TOF
• Mass spectrometry
• PCR
• Diagnostic accuracy

• All authors: Prehn, J. van; Veen, S.Q. van; Schelfaut, J.J.G.; Wessels, E.
• Date: 2016-05-01
• Journal: Diagnostic Microbiology and Infectious Disease
• Volume: 85
• Issue: 1
• Pages: 9 - 11
• DOI: doi:10.1016/j.diagmicrobio.2016.01.012