Leiden University Scholarly Publications

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Tientcheu, L.D.; Haks, M.C.; Agbla, S.C.; Sutherland, J.S.; Adetifa, I.M.; Donkor, S.; ... ; Ota, M.O. (2016)

Host Immune Responses Differ between M-africanum- and M-tuberculosis-Infected Patients following Standard Anti-tuberculosis Treatment

Article / Letter to editor
Epidemiological differences exist between Mycobacterium africanum (Maf)- and Mycobacterium tuberculosis (Mtb)-infected patients, but to date, contributing host factors have not been characterised. We analysed clinical outcomes, as well as soluble markers and gene expression profiles in unstimulated, and ESAT6/CFP-10-, whole-Maf- and Mtb-stimulated blood samples of 26 Maf- and 49 Mtb-HIV-negative tuberculosis patients before, and after 2 and 6 months of anti-tuberculosis therapy. Before treatment, both groups had similar clinical parameters, but differed in few cytokines concentration and gene expression profiles. Following treatment the body mass index, skinfold thickness and chest X-ray scores showed greater improvement in the Mtb-compared to Maf-infected patients, after adjusting for age, sex and ethnicity (p = 0.02; 0.04 and 0.007, respectively). In addition, in unstimulated blood, IL-12p70, IL12A and TLR9 were significantly higher in Maf-infected patients, while IL-15, IL-8 and...Show moreEpidemiological differences exist between Mycobacterium africanum (Maf)- and Mycobacterium tuberculosis (Mtb)-infected patients, but to date, contributing host factors have not been characterised. We analysed clinical outcomes, as well as soluble markers and gene expression profiles in unstimulated, and ESAT6/CFP-10-, whole-Maf- and Mtb-stimulated blood samples of 26 Maf- and 49 Mtb-HIV-negative tuberculosis patients before, and after 2 and 6 months of anti-tuberculosis therapy. Before treatment, both groups had similar clinical parameters, but differed in few cytokines concentration and gene expression profiles. Following treatment the body mass index, skinfold thickness and chest X-ray scores showed greater improvement in the Mtb-compared to Maf-infected patients, after adjusting for age, sex and ethnicity (p = 0.02; 0.04 and 0.007, respectively). In addition, in unstimulated blood, IL-12p70, IL12A and TLR9 were significantly higher in Maf-infected patients, while IL-15, IL-8 and MIP-1 alpha were higher in Mtb-infected patients. Overnight stimulation with ESAT-6/CFP-10 induced significantly higher levels of IFN-gamma and TNF-alpha production, as well as gene expression of CCL4, IL1B and TLR4 in Mtb-compared to Maf-infected patients. Our study confirms differences in clinical features and immune genes expression and concentration of proteins associated with inflammatory processes between Mtb- and Maf-infected patients following anti-tuberculosis treatment These findings have public health implications for treatment regimens, and biomarkers for tuberculosis diagnosis and susceptibility.Show less
All authors
Tientcheu, L.D.; Haks, M.C.; Agbla, S.C.; Sutherland, J.S.; Adetifa, I.M.; Donkor, S.; Quinten, E.; Daramy, M.; Antonio, M.; Kampmann, B.; Ottenhoff, T.H.M.; Dockrell, H.M.; Ota, M.O.
Date
2016-05-01
Journal
PLoS Neglected Tropical Diseases
Volume
10
Issue
5
DOI
doi:10.1371/journal.pntd.0004701