Leiden University Scholarly Publications

Persistent URL of this record https://hdl.handle.net/1887/83300

In Collections

This item can be found in the following collections:

Jónasdóttir, H.S. (2020)

New technologies for the analysis of oxylipids : role and metabolism of oxylipids in rheumatic diseases

Doctoral Thesis
The focus of this thesis is (oxy)lipid analysis. An introductory overview is given of lipids, lipidomics, and lipid mediators in inflammation; and in subsequent chapters the focus is on development of lipidomic methods for the analysis of oxidized lipids. This touches on different extraction methods of the (target) analytes, sample handling/preparation and storage, separation techniques, and finally the MS detection/resolution/ specificity etc. These methods are then utilized, mainly on human plasma and synovial fluid (SF) samples. Special focus is on the targeted analysis of hydroxylated fatty acids (hFAs), specialized pro-resolving mediators (SPMs) and their intermediates, and other oxylipids present in inflammation and its resolution in rheumatic diseases. For example, the bioactivity of oxylipids and LMs is highly stereospecific, and some difficulties have arisen in the chromatographic separation and resolution of these stereoisomers using classical reversed phase liquid...Show more The focus of this thesis is (oxy)lipid analysis. An introductory overview is given of lipids, lipidomics, and lipid mediators in inflammation; and in subsequent chapters the focus is on development of lipidomic methods for the analysis of oxidized lipids. This touches on different extraction methods of the (target) analytes, sample handling/preparation and storage, separation techniques, and finally the MS detection/resolution/ specificity etc. These methods are then utilized, mainly on human plasma and synovial fluid (SF) samples. Special focus is on the targeted analysis of hydroxylated fatty acids (hFAs), specialized pro-resolving mediators (SPMs) and their intermediates, and other oxylipids present in inflammation and its resolution in rheumatic diseases. For example, the bioactivity of oxylipids and LMs is highly stereospecific, and some difficulties have arisen in the chromatographic separation and resolution of these stereoisomers using classical reversed phase liquid chromatography tandem MS (RPLC-MS/MS), perhaps sometimes resulting in wrong conclusions being drawn, one isomer being confused with another. The work in this thesis was therefore focused on further development and application of analysis platforms for oxidized lipids; their identification, separation and levels in different matrices. All of which, work that can be further used in inflammatory or rheumatoid research. Show less
Oxylipids
Specialized pro-resolving lipid mediators
Lipidomics
LC-MS/MS
Stability
Storage
Ion mobility
Inflammation
Rheumatic diseases
All authors
Jónasdóttir, H.S.
Supervisor
Toes, R.E.M.
Co-supervisor
Giera, M.A.; Ioan-Fascinay, A.
Committee
Huizinga, T.W.J.; Koning, F.; O'Donnell, V.; Bobeldijk, I.
Qualification
Doctor (dr.)
Awarding Institution
Faculty of Medicine, Leiden University Medical Center (LUMC), Leiden University
Date
2020-01-23
ISBN (print)
9789463757560

Funding

Sponsorship
The work of HSJ was supported by the Jan Veltkamp fonds (LUMC)