Solid state protein monolayers: morphological, conformational, and functional properties

Pompa, P.P.; Biasco, A.; Frascerra, V.; Calabi, F.; Cingolani, R.; Rinaldi, R.; ... ; Canters, G.W. (2004)

Article / Letter to editor

We have studied the morphological, conformational, and electron-transfer (ET) function of the metalloprotein azurin in the solid state, by a combination of physical investigation methods, namely atomic force microscopy, intrinsic fluorescence spectroscopy, and scanning tunneling microscopy. We demonstrate that a “solid state protein film” maintains its nativelike conformation and ET function, even after removal of the aqueous solvent.

• All authors: Pompa, P.P.; Biasco, A.; Frascerra, V.; Calabi, F.; Cingolani, R.; Rinaldi, R.; Verbeet, M.P.; Waal, E. de; Canters, G.W.
• Date: 2004-11-15
• Journal: Journal of Chemical Physics
• Volume: 121
• Issue: 21
• Pages: 10325 - 10328
• DOI: doi:10.1063/1.1828038