Clinical glycomics of antibodies by mass spectometry

Haan, N. de (2019)

Doctoral Thesis

In this thesis, methods were developed, and antibody glycosylation was characterized in order to further the clinical application of antibody glycosylation analysis. New mass spectrometric workflows were introduced in Chapters 2 and 8. Chapter 7 showed the differential characteristics of murine IgG glycosylation of different strains and highlighted the profound differences between humans and mice, with regard to IgG glycosylation. Chapters 4 and 8 showed the use of controlled human situations to study the regulatory mechanisms of IgG and IgA glycosylation. Finally, Chapters 3, 5 and 6, identified glycosidic differences with specified (patho)physiological conditions, which might be exploited for patient stratification in the future.

• Glycomics
• Antibody
• MALDI-TOF-MS
• LC-MS
• Sialic acids
• Saliva
• Plasma
• Murine glycosylation
• Biomarker

• All authors: Haan, N. de
• Supervisor: Wuhrer, M.
• Co-supervisor: Falck, D.
• Committee: Lankester, A.C.; Cobbaert, C.M.; Boons, G.-J.; Vidarsson, G.
• Qualification: Doctor (dr.)
• Awarding Institution: Medicine / Leiden University Medical Center (LUMC), Leiden University
• Date: 2019-02-14
• ISBN (print): 9789463752367
• Sponsorship: European Union Seventh Framework Program IBD-BIOM, grant number: 305479