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- Title page_Table of contentst
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- Chapter 1 Introduction
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- Chapter 7 General conclusion and outlook
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- References
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Applications of paramagnetic NMR spectroscopy for protein research
challenging systems in liquid-state NMR using paramagnetic effects generated by the
two-armed probe CLaNP-5. Chapter 1 is an introduction about NMR assignment
development and challenges and a brief opening to the theory of paramagnetic effects
and their applications. Chapter 2 describes the upgrade of PARAssign software and its
ability to assign and assess methyl groups of a 25 kDa protein using experimental
pseudocontact shifts from 1 to 3 paramagnetic centers. Chapter 3 describes the
implementation of residual dipolar coupling into PARAssign software as extra dataset
input in addition to the pseudocontact shifts. Applications are given for residue
selective isotope labeled protein. Chapter 4 depicts the use of pseudocontact shifts
used to probe small methyl group movements and re-orientation under ligand binding.
Chapter 5 gives some clues about...Show moreThe aim of the research presented in this thesis was to develop new methods for
challenging systems in liquid-state NMR using paramagnetic effects generated by the
two-armed probe CLaNP-5. Chapter 1 is an introduction about NMR assignment
development and challenges and a brief opening to the theory of paramagnetic effects
and their applications. Chapter 2 describes the upgrade of PARAssign software and its
ability to assign and assess methyl groups of a 25 kDa protein using experimental
pseudocontact shifts from 1 to 3 paramagnetic centers. Chapter 3 describes the
implementation of residual dipolar coupling into PARAssign software as extra dataset
input in addition to the pseudocontact shifts. Applications are given for residue
selective isotope labeled protein. Chapter 4 depicts the use of pseudocontact shifts
used to probe small methyl group movements and re-orientation under ligand binding.
Chapter 5 gives some clues about how paramagnetic effect such as RDC but also PCS
can provide information for challenging highly dynamic system. Chapter 6 gives
insights for PCS application to very large multimeric protein, the challenges and
limitations are discussed. Chapter 7 provides a discussion and few perspectives about
the work carried out and presented in this thesis.Show less
- All authors
- Lescanne, M.
- Supervisor
- Ubbink, M.; Luchinat, C.
- Committee
- Groot, H.J.M. de; Overkleeft, H.S.; Marion, D.; Pandit, A.; Liu, W.M.
- Qualification
- Doctor (dr.)
- Awarding Institution
- Leiden Institute of Chemistry (LIC) , Science , Leiden University
- Date
- 2018-05-29
- ISBN (print)
- 9789491546150