New players in zebrafish caudal fin regeneration : differential requirement for protein-tyrosine phosphatases

Hale, A.J.M. (2017)

Doctoral Thesis

Hydrogen peroxide (H2O2) is produced following injury and is required for regeneration. However, how H2O2 coordinates multiple signalling pathways required for regeneration is not known. Protein-tyrosine phosphatases (PTPs) are regulators of signal transduction, reversing the phosphorylation of substrates, and are essential for many cellular processes. PTPs are also highly sensitive to reversible oxidation-mediated inhibition by H2O2.

My research identified that some, but not all, PTPs become oxidized following amputation of the zebrafish caudal fin, and implicates PTPs that are oxidized as necessary for the regenerative process. Furthermore, I delineated that the PTPs Shp2 and Pten are required for the regenerative outgrowth phase of regeneration, and that Shp2 and Pten have a role in driving MAPK signalling and balancing PI3K signalling, respectively, during this phase.

• Zebrafish
• Protein-tyrosine Phosphatases
• Regeneration
• Caudal fin
• Oxidation

• All authors: Hale, A.J.M.
• Supervisor: Hertog, J. den; Spaink, H.P.
• Committee: Wezel, G.P. van; Meijer, A.H.; Richardson, M.K.; Bakkers, J.; Hurlstone, A.F.
• Qualification: Doctor (dr.)
• Awarding Institution: Institute of Biology, Science, Leiden University
• Date: 2017-10-19
• ISBN (print): 9789462337336