This thesis investigates the immunological effects of mesenchymal stromal cell (MSC) therapy in the context of kidney transplantation, with the intent to improve long-term graft survival and reduce the need for nephrotoxic immunosuppressive drugs. Technical innovations were first established by optimising mass cytometry protocols, including live-cell barcoding and the use of frozen antibody mixes to ensure reproducibility and high-quality single-cell analysis.
Subsequent studies explored immune dynamics following both autologous and allogenic MSC infusion in kidney transplant recipients. Using high-dimensional single-cell analyses, MSC therapy was shown to induce significant alterations in peripheral immune cell subsets. Remarkably, rapid immune changes in B and T cell phenotypes were observed as early as four hours post MSC infusion.
The thesis also examined the role of indoleamine 2,3-dioxygenase (IDO) expression in glomerular endothelial cells of...
Show moreThis thesis investigates the immunological effects of mesenchymal stromal cell (MSC) therapy in the context of kidney transplantation, with the intent to improve long-term graft survival and reduce the need for nephrotoxic immunosuppressive drugs. Technical innovations were first established by optimising mass cytometry protocols, including live-cell barcoding and the use of frozen antibody mixes to ensure reproducibility and high-quality single-cell analysis.
Subsequent studies explored immune dynamics following both autologous and allogenic MSC infusion in kidney transplant recipients. Using high-dimensional single-cell analyses, MSC therapy was shown to induce significant alterations in peripheral immune cell subsets. Remarkably, rapid immune changes in B and T cell phenotypes were observed as early as four hours post MSC infusion.
The thesis also examined the role of indoleamine 2,3-dioxygenase (IDO) expression in glomerular endothelial cells of transplanted kidneys with glomerulitis, revealing its association with local immune activation.
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