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- Experimental_Dermatology_-_2023_-_Raktoe
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Mimicking fat grafting of fibrotic scars using 3D-organotypic skin cultures: fat grafting in 3D organotypic skin model
Wound healing of deep burn injuries is often accompanied with severe scarring, such as hypertrophic scar (HTS) formation. In severe burn wounds, where the subcutis is also damaged, the scars adhere to structures underneath, resulting in stiffness of the scar and impaired motion. Over the recent years, a promising solution has emerged: autologous fat grafting, also known as lipofilling. Previous clinical reports have shown that the antifibrotic effect has been attributed due to the presence of adipose-derived stromal cells (ADSC).
In the proposed study we aim to investigate the effect of fat grafting in 3D organotypic skin cultures mimicking an HTS-like environment.
To this end, organotypic skin cultures were embedded with normal skin fibroblasts (NF) or HTS-derived fibroblasts with or without incorporation of human adipose subcutaneous tissue (ADT) and one part was thermally wounded to examine their effect on epithelialisation. The developed skin cultures...
Show moreWound healing of deep burn injuries is often accompanied with severe scarring, such as hypertrophic scar (HTS) formation. In severe burn wounds, where the subcutis is also damaged, the scars adhere to structures underneath, resulting in stiffness of the scar and impaired motion. Over the recent years, a promising solution has emerged: autologous fat grafting, also known as lipofilling. Previous clinical reports have shown that the antifibrotic effect has been attributed due to the presence of adipose-derived stromal cells (ADSC).
In the proposed study we aim to investigate the effect of fat grafting in 3D organotypic skin cultures mimicking an HTS-like environment.
To this end, organotypic skin cultures were embedded with normal skin fibroblasts (NF) or HTS-derived fibroblasts with or without incorporation of human adipose subcutaneous tissue (ADT) and one part was thermally wounded to examine their effect on epithelialisation. The developed skin cultures were analysed on morphology and protein level.
Analysis revealed that ADT-containing organotypic skin cultures comprise an improved epidermal homeostasis, and a fully formed basement membrane, similar to native human skin (NHS). Furthermore, addition of ADT significantly reduced myofibroblast presence, which indicates its anti-fibrotic effect. Finally, re-epithelialization measurements showed that ADT reduced re-epithelialization in skin cultures embedded with NFs, whereas HTS-fibroblast-embedded skin cultures showed complete wound closure.
In conclusion, we succeeded in developing a 3D organotypic HTS-skin model incorporated with subcutaneous tissue that allows further investigation on the molecular mechanism of fat grafting.
Show less- All authors
- Raktoe, R.; Kwee, A.K.A.L.; Rietveld, M.; Marsidi, N.; Genders, R.; Quint, K.; Doorn, R. van; Zuijlen, P. van; Ghalbzouri, A.E.L.
- Date
- 2023
- Journal
- Experimental Dermatology
- Volume
- 32
- Issue
- 10
- Pages
- 1752 - 1762
Funding
- Grant number
- 14.105