Clinicogenomic associations in childhood Langerhans cell histiocytosis: an international cohort study

Kemps, P.G.; Zondag, T.C.E.; Arnardóttir, H.B.; Solleveld-Westerink, N.; Borst, J.; Steenwijk, E.C.; Egmond, D. van; Swennenhuis, J.F.; Stelloo, E.; Trambusti, I.; Verdijk, R.M.; Noesel, C.J.M. van; Cleven, A.H.G.; Scheijde-Vermeulen, M.A.; Koudijs, M.J.; Krsková, L.; Hawkins, C.; Egeler, R.M.; Brok, J.; Greenwood, T.V.; Svoijr, K.; Beishuizen, A.; Laar, J.A.M. van; Pötschger, U.; Hutter, C.; Sieni, E.; Minkov, M.; Abla, O.; Wezel, T. van; Bos, C. van den; Halteren, A.G.S. van

doi:doi:10.1182/bloodadvances.2022007947

Langerhans cell histiocytosis (LCH) is a rare neoplastic disorder caused by somatic genetic alterations in hematopoietic precursor cells differentiating into CD1a⁺/CD207⁺ histiocytes. LCH clinical manifestation is highly heterogeneous. BRAF and MAP2K1 mutations account for ∼80% of genetic driver alterations in neoplastic LCH cells. However, their clinical associations remain incompletely understood. Here, we present an international clinicogenomic study of childhood LCH, investigating 377 patients genotyped for at least BRAF^V600E. MAPK pathway gene alterations were detected in 300 (79.6%) patients, including 191 (50.7%) with BRAF^V600E, 54 with MAP2K1 mutations, 39 with BRAF exon 12 mutations, 13 with rare BRAF alterations, and 3 with ARAF or KRAS mutations. Our results confirm that BRAF^V600E associates with...Show more Langerhans cell histiocytosis (LCH) is a rare neoplastic disorder caused by somatic genetic alterations in hematopoietic precursor cells differentiating into CD1a⁺/CD207⁺ histiocytes. LCH clinical manifestation is highly heterogeneous. BRAF and MAP2K1 mutations account for ∼80% of genetic driver alterations in neoplastic LCH cells. However, their clinical associations remain incompletely understood. Here, we present an international clinicogenomic study of childhood LCH, investigating 377 patients genotyped for at least BRAF^V600E. MAPK pathway gene alterations were detected in 300 (79.6%) patients, including 191 (50.7%) with BRAF^V600E, 54 with MAP2K1 mutations, 39 with BRAF exon 12 mutations, 13 with rare BRAF alterations, and 3 with ARAF or KRAS mutations. Our results confirm that BRAF^V600E associates with lower age at diagnosis and higher prevalence of multisystem LCH, high-risk disease, and skin involvement. Furthermore, BRAF^V600E appeared to correlate with a higher prevalence of central nervous system (CNS)–risk bone lesions. In contrast, MAP2K1 mutations associated with a higher prevalence of single-system (SS)-bone LCH, and BRAF exon 12 deletions seemed to correlate with more lung involvement. Although BRAF^V600E correlated with reduced event-free survival in the overall cohort, neither BRAF nor MAP2K1 mutations associated with event-free survival when patients were stratified by disease extent. Thus, the correlation of BRAF^V600E with inferior clinical outcome is (primarily) driven by its association with disease extents known for high rates of progression or relapse, including multisystem LCH. These findings advance our understanding of factors underlying the remarkable clinical heterogeneity of LCH but also question the independent prognostic value of lesional BRAF^V600E status.
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Clinicogenomic associations in childhood Langerhans cell histiocytosis: an international cohort study