Multiple reaction monitoring assays for large-scale quantitation of proteins from 20 mouse organs and tissues

Michaud, S.A.; Petrosová, H.; Sinclair, N.J.; Kinnear, A.L.; Jackson, A.M.; McGuire, J.C.; ... ; Borchers, C.H. (2024)

Article / Letter to editor

Mouse is the mammalian model of choice to study human health and disease due to its size, ease of breeding and the natural occurrence of conditions mimicking human pathology. Here we design and validate multiple reaction monitoring mass spectrometry (MRM-MS) assays for quantitation of 2118 unique proteins in 20 murine tissues and organs. We provide open access to technical aspects of these assays to enable their implementation in other laboratories, and demonstrate their suitability for proteomic profiling in mice by measuring normal protein abundances in tissues from three mouse strains: C57BL/6NCrl, NOD/SCID, and BALB/cAnNCrl. Sex- and strain-specific differences in protein abundances are identified and described, and the measured values are freely accessible via our MouseQuaPro database: http://mousequapro.proteincentre.com. Together, this large library of quantitative MRM-MS assays established in mice and the measured baseline protein abundances represent an important resource for research involving mouse models.Development of MRM-MS assays for 2118 proteins in 20 mouse tissues enables accurate quantitation. Protein concentrations measured from 3 mouse strains using these assays demonstrate proteomic phenotyping and are provided in an online database.

• All authors: Michaud, S.A.; Petrosová, H.; Sinclair, N.J.; Kinnear, A.L.; Jackson, A.M.; McGuire, J.C.; Hardie, D.B.; Bhowmick, P.; Ganguly, M.; Flenniken, A.M.; Nutter, L.M.J.; McKerlie, C.; Smith, D.; Mohammed, Y.; Schibli, D.; Sickmann, A.; Borchers, C.H.
• Date: 2024-01-02
• Journal: Communications Biology
• Volume: 7
• Issue: 1
• DOI: doi:10.1038/s42003-023-05687-0