Application of mismatch detection methods in DNA computing

Henkel, C.V.; Rozenberg, G.; Spaink, H.P. (2006)

Article / Letter to editor

In many implementations of DNA computing, reliable detection of hybridization is of prime importance. We have applied several well-established DNA mutation scanning methods to this problem. Since they have been developed for speed and accuracy, these technologies are very promising for DNA computing. We have benchmarked a heteroduplex migration assay and enzymatic detection of mismatches on a 4 variable instance of 3SAT, using a previously described blocking algorithm. The first method is promising, but yielded ambiguous results. On the other hand, we were able to distinguish all perfect from imperfect duplexes by means of a CEL I mismatch endonuclease assay.

• All authors: Henkel, C.V.; Rozenberg, G.; Spaink, H.P.
• Date: 2006
• Journal: Natural computing
• Volume: 5
• Pages: 151 - 163
• DOI: doi:10.1007/s11047-005-4463-z