A Lotus japonicus Nodulation System Based on Heterologous Expression of the Fucosyl Transferase NodZ and the Acetyl Transferase NolL in Rhizobium leguminosarum

Bras, C.P.; Jordá, M.A.; Wijfjes, A.H.M.; Harteveld, M.; Stuurman, N.; Thomas-Oates, J.E; Spaink, H.P. (2000)

Article / Letter to editor

Heterologous expression of NodZ and NoIL proteins in Rhizobium leguminosarum by. viciae led to the production of acetyl fucosylated lipo-chitin oligosaccharides (LCOs), indicating that the NoIL protein obtained from Mesorhizobium loti functions as an acetyl transferase. We show that the NoIL-dependent acetylation is specific for the fucosyl penta-N-acetylglucosamine species. In addition, the NoIL protein caused elevated production of LCOs, Efficient nodulation of Lotus japonicus by the NodZ/NoIL-producing strain was demonstrated. Nodulation efficiency was further improved by the addition of the ethylene inhibitor L-alpha-(2-aminoethoxyvinyl) glycine (AVG).

• All authors: Bras, C.P.; Jordá, M.A.; Wijfjes, A.H.M.; Harteveld, M.; Stuurman, N.; Thomas-Oates, J.E; Spaink, H.P.
• Date: 2000-04-01
• Journal: Molecular Plant-Microbe Interactions
• Volume: 13
• Issue: 4
• Pages: 475 - 479
• DOI: doi:10.1094/MPMI.2000.13.4.475