Persistent URL of this record https://hdl.handle.net/1887/3665348
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Engineering of antigen-saving dissolving microneedles for intradermal vaccine delivery
Currently, the most frequently used fabrication approach (centrifugation method) results in major antigen waste. As antigens are generally very expensive, the current method is not suitable for the cost-effective fabrication of dMNAs. Therefore, a novel dMNA production technology that lowers antigen loss is required. In the thesis, the engineering of a novel dMNA production system based on a dispensing approach that minimizes antigen loss was presented. Furthermore, a comparison was made between the two production approaches (centrifugation and dispensing methods) for vaccine delivery via dMNAs. In additional studies, this novel method has been used to deliver influenza vaccine into the skin leading successfully to a high and protective immune response. In conclusion, a dispensing system for dMNAs production, as opposed to the centrifugation process, is a promising method for the cost-effective fabrication of dMNAs.
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- All authors
- Lee, J.
- Supervisor
- Bouwstra, J.; Jiskoot, W.†
- Co-supervisor
- Maaden, K. van der
- Committee
- Irth, H.; Lange, E.C.M. de; Foged, C.; Arens, R.; Rissmann, R.; Kersten, G.F.A,
- Qualification
- Doctor (dr.)
- Awarding Institution
- Leiden Academic Centre for Drug Research (LACDR) , Faculty of Science , Leiden University
- Date
- 2023-11-29
- ISBN (print)
- 9789493289352