Chromatin accessibility landscapes activated by cell-surface and intracellular immune receptors

Ding, P.; Sakai, T.; Krishna, S.R.; Manosalva, P.N.; Guo, W.; Ngou, B.P.K.; ... ; Jones, J.D.G. (2021)

Article / Letter to editor

Activation of cell-surface and intracellular receptor-mediated immunity results in rapid transcriptional reprogramming that underpins disease resistance. However, the mechanisms by which co-activation of both immune systems lead to transcriptional changes are not clear. Here, we combine RNA-seq and ATAC-seq to define changes in gene expression and chromatin accessibility. Activation of cell-surface or intracellular receptor-mediated immunity, or both, increases chromatin accessibility at induced defence genes. Analysis of ATAC-seq and RNA-seq data combined with publicly available information on transcription factor DNA-binding motifs enabled comparison of individual gene regulatory networks activated by cell-surface or intracellular receptor-mediated immunity, or by both. These results and analyses reveal overlapping and conserved transcriptional regulatory mechanisms between the two immune systems.

• All authors: Ding, P.; Sakai, T.; Krishna, S.R.; Manosalva, P.N.; Guo, W.; Ngou, B.P.K.; He, S.; Liu, C.; Feng, X.; Zhang, R.; Vandepoele, K.; MacLean, D.; Jones, J.D.G.
• Date: 2021-08-13
• Journal: Journal of Experimental Botany
• Volume: 72
• Issue: 22
• Pages: 7927 - 7941
• DOI: doi:10.1093/jxb/erab373