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Jacquier, H.; Vironneau, P.; Dang, H.; Verillaud, B.; Lamers, G.E.M.; Herman, P.; ... ; Karnia, R. (2020)

Bacterial biofilm in adenoids of children with chronic otitis media: part II: a case–control study of nasopharyngeal microbiota, virulence, and resistance of biofilms in adenoids

Article / Letter to editor
Background: We previously described that adenoid tissue in children with chronic otitis media (COM) contained more mucosal biofilms than adenoid tissue removed for hypertrophy.Aims/objectives: The aim of the second part was to characterize nasopharyngeal microbiota and explore virulence of the most common middle ear pathogens.Material and methods: Bacteriological analysis was performed following a culture-based approach on the samples recovered from 30 patients of COM group (15 biofilm-positive and 15 biofilm-negative) and from 30 patients of a control group (15 biofilm-positive and 15 biofilm-negative). Virulence factors of Streptococcus pneumoniae, Streptococcus pyogenes, and Haemophilus influenzae were investigated.Results: The most frequent species were Firmicutes followed by Proteobacteria and Actinobacteria. The presence of biofilm was statistically associated with an increase of the number of bacterial species and...Show moreBackground: We previously described that adenoid tissue in children with chronic otitis media (COM) contained more mucosal biofilms than adenoid tissue removed for hypertrophy.Aims/objectives: The aim of the second part was to characterize nasopharyngeal microbiota and explore virulence of the most common middle ear pathogens.Material and methods: Bacteriological analysis was performed following a culture-based approach on the samples recovered from 30 patients of COM group (15 biofilm-positive and 15 biofilm-negative) and from 30 patients of a control group (15 biofilm-positive and 15 biofilm-negative). Virulence factors of Streptococcus pneumoniae, Streptococcus pyogenes, and Haemophilus influenzae were investigated.Results: The most frequent species were Firmicutes followed by Proteobacteria and Actinobacteria. The presence of biofilm was statistically associated with an increase of the number of bacterial species and Firmicutes phylum regardless of the condition (case/control). No virulence factors associated with invasive isolates were found for the most common middle ear pathogens.Conclusions and significance: This case-control study demonstrated that the presence of COM plus biofilm was associated with a given microbiota which contained more Firmicutes. Our study allows a better understanding of physiopathological mechanisms involved in chronic otitis media and paves the way for further investigations.Show less
All authors
Jacquier, H.; Vironneau, P.; Dang, H.; Verillaud, B.; Lamers, G.E.M.; Herman, P.; Vicaut, E.; Tessier, N.; Bidet, P.; Varon, E.; Abbeele, T. van den; Cambau, E.; Bercot, B.; Karnia, R.
Date
2020-02-12
Journal
Acta Oto-laryngologica
Volume
140
Issue
3
Pages
220 - 224
DOI
doi:10.1080/00016489.2020.1718749