Leiden University Scholarly Publications

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Berni, F. (2023)

Synthesis and applications of cell wall glycopolimer fragments from Staphilococci and Enterococci

Doctoral Thesis
Carbohydrates are present on the surface of bacteria making them suitable antigen candidates for vaccine development. This thesis deals with the synthesis of two carbohydrate-based components; the capsular polisaccharide of S. aureus type 5 and teichoic acids from staphilococci and enterococci species. The synthesis of well-defined structures can allow an in-depth characterization of antigen-antibody binding that ultimately can help the development of robust and high effective vaccine. In the case of teichoic acids for example, it has been introduced the application of a microarray technology to streamline the preferential binding of immune proteins from different sources. The binding towards teichoic acids like structures has been further explore by developing a monoclonal antibody starting from a fully sinthetic glycoconjugate. Different techniques have been used which in conclusion suggest an indirect contribution of the glycosidic appandage to the binding towards the glycerol...Show moreCarbohydrates are present on the surface of bacteria making them suitable antigen candidates for vaccine development. This thesis deals with the synthesis of two carbohydrate-based components; the capsular polisaccharide of S. aureus type 5 and teichoic acids from staphilococci and enterococci species. The synthesis of well-defined structures can allow an in-depth characterization of antigen-antibody binding that ultimately can help the development of robust and high effective vaccine. In the case of teichoic acids for example, it has been introduced the application of a microarray technology to streamline the preferential binding of immune proteins from different sources. The binding towards teichoic acids like structures has been further explore by developing a monoclonal antibody starting from a fully sinthetic glycoconjugate. Different techniques have been used which in conclusion suggest an indirect contribution of the glycosidic appandage to the binding towards the glycerol-phosphate backbone.
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Structure-immune relationship
Synthetic teichoic acid based antigens
Synthetic CP5 antigen
Teichoic acids microarray
Serum screening
Antibody-carbohydrate binding
All authors
Berni, F.
Supervisor
Codée, J.D.C.
Co-supervisor
Marel, G.A. van der
Committee
Ubbink, M.; Overkleeft, H.S.; Filippov, D.V.; Sorge, N. van; Jiménez Barbero, J.
Qualification
Doctor (dr.)
Awarding Institution
Leiden Institute of Chemistry (LIC), Faculty of Science, Leiden University
Date
2023-10-19