An ENDOR and ESEEM study of the blue copper protein azurin

Coremans, J.W.A.; Gastel, M. van; Poluektov, O.G.; Groenen, E.J.J.; Blaauwen, T. den; Pouderoyen, G. van; ... ; Messerschmidt, A. (1995)

Article / Letter to editor

We report electron-nuclear double-resonance experiments on a single crystal of azurin at 95 GHz and electron-spin-echo envelope-modulation experiments on frozen solutions of azurin and of the H117G mutant at 9 GHz. The hyperfine and quadrupole tensors of the two remote nitrogens of the histidine ligands of copper are assigned and discussed. A third nucleus is found to contribute to the echo-modulation spectrum and this probably concerns an amide nitrogen of the peptide backbone.

• All authors: Coremans, J.W.A.; Gastel, M. van; Poluektov, O.G.; Groenen, E.J.J.; Blaauwen, T. den; Pouderoyen, G. van; Canters, G.W.; Nar, H.; Hammann, C.; Messerschmidt, A.
• Date: 1995-03-24
• Journal: Chemical Physics Letters
• Volume: 235
• Issue: 3-4
• Pages: 202 - 210
• DOI: doi:10.1016/0009-2614(95)00114-J
• Link: https://www.sciencedirect.com/science/article/pii/000926149500114J?via%3...