CD45RB glycosylation and Ig isotype define maturation of functionally distinct B cell subsets in human peripheral blood

Koers, J.; Pollastro, S.; Tol, S.; Pico-Knijnenburg, I.; Derksen, N.I.L.; Schouwenburg, P.A. van; ... ; Rispens, T. (2022)

Article / Letter to editor

Glycosylation of CD45RB (RB+) has recently been identified to mark antigen-experienced B cells, independent of their CD27 expression. By using a novel combination of markers including CD45RB glycosylation, CD27 and IgM/IgD isotype expression we segregated human peripheral blood B cell subsets and investigated their IGHV repertoire and in vitro functionality. We observed distinct maturation stages for CD27-RB+ cells, defined by differential expression of non-switched Ig isotypes. CD27-RB+ cells, which only express IgM, were more matured in terms of Ig gene mutation levels and function as compared to CD27-RB+ cells that express both IgM and IgD or cells that were CD27-RB-. Moreover, CD27-RB+IgM+ cells already showed remarkable rigidity in IgM isotype commitment, different from CD27-RB+IgMD+ and CD27-RB- cells that still demonstrated great plasticity in B cell fate decision. Thus, glycosylation of CD45RB is indicative for antigen-primed B cells, which are, dependent on the Ig isotype, functionally distinct.

• CD45RB glycosylation
• CD27
• B cell subset heterogeneity
• naive B cells
• memory B cells

• All authors: Koers, J.; Pollastro, S.; Tol, S.; Pico-Knijnenburg, I.; Derksen, N.I.L.; Schouwenburg, P.A. van; Burg, M. van der; Ham, S.M. van; Rispens, T.
• Date: 2022-04-28
• Journal: Frontiers in Immunology
• Volume: 13
• DOI: doi:10.3389/fimmu.2022.891316