Intraoperative near-infrared fluorescence imaging with Indocyanine green for identification of gastrointestinal stromal Tumors (GISTs), a feasibility study

Kalisvaart, G.M.; Meijer, R.P.J.; Bijlstra, O.D.; Galema, H.A.; Steur, W.O. de; Hartgrink, H.H.; ... ; Hage, J.A. van der (2022)

Article / Letter to editor

Simple Summary: Surgical resection plays a pivotal role in the treatment of GIST patients. The current study aims to explore the use of near-infrared fluorescence imaging to optimize the intraoperative tumor identification of GISTs. For this purpose, the potential and limitations of the widely used, and non-specific, tracer indocyanine green were assessed in a multicenter study including 10 patients. Our results show that GISTs typically have similar fluorescence intensity to the surrounding tissue, within several minutes after the intravenous administration of indocyanine green. These findings justify future research into specific fluorescent tracers for GISTs, and set a reference for future intraoperative imaging trials. Background: Optimal intraoperative tumor identification of gastrointestinal stromal tumors (GISTs) is important for the quality of surgical resections. This study aims to assess the potential of near-infrared fluorescence (NIRF) imaging with indocyanine green (ICG) to improve intraoperative tumor identification. Methods: Ten GIST patients, planned to undergo resection, were included. During surgery, 10 mg of ICG was intravenously administered, and NIRF imaging was performed at 5, 10, and 15 min after the injection. The tumor fluorescence intensity was visually assessed, and tumor-to-background ratios (TBRs) were calculated for exophytic lesions. Results: Eleven GIST lesions were imaged. The fluorescence intensity of the tumor was visually synchronous and similar to the background in five lesions. In one lesion, the tumor fluorescence was more intense than in the surrounding tissue. Almost no fluorescence was observed in both the tumor and healthy peritoneal tissue in two patients with GIST lesions adjacent to the liver. In three GISTs without exophytic growth, no fluorescence of the tumor was observed. The median TBRs at 5, 10, and 15 min were 1.0 (0.4-1.2), 1.0 (0.5-1.9), and 0.9 (0.7-1.2), respectively. Conclusion: GISTs typically show similar fluorescence intensity to the surrounding tissue in NIRF imaging after intraoperative ICG administration. Therefore, intraoperatively administered ICG is currently not applicable for adequate tumor identification, and further research should focus on the development of tumor-specific fluorescent tracers for GISTs.

• gastrointestinal stromal tumor (GIST)
• near-infrared fluorescence imaging
• image-guided surgery
• indocyanine green (ICG)

• All authors: Kalisvaart, G.M.; Meijer, R.P.J.; Bijlstra, O.D.; Galema, H.A.; Steur, W.O. de; Hartgrink, H.H.; Verhoef, C.; Geus-Oei, L.F. de; Grunhagen, D.J.; Schrage, Y.M.; Vahrmeijer, A.L.; Hage, J.A. van der
• Date: 2022-03-01
• Journal: Cancers
• Volume: 14
• Issue: 6
• DOI: doi:10.3390/cancers14061572