Microphysiological stem cell models of the human heart

Arslan, U.; Moruzzi, A.; Nowacka, J.; Mummery, C.L.; Eckardt, D.; Loskill, P.; Orlova, V.V. (2022)

Article / Letter to editor

Models of heart disease and drug responses are increasingly based on human pluripotent stem cells (hPSCs) since their ability to capture human heart (dys-)function is often better than animal models. Simple monolayer cultures of hPSC-derived cardiomyocytes, however, have shortcomings. Some of these can be overcome using more complex, multi cell-type models in 3D. Here we review modalities that address this, describe efforts to tailor readouts and sensors for monitoring tissue- and cell physiology (exogenously and in situ) and discuss perspectives for implementation in industry and academia.

• Human induced pluripotent stem cells
• Cardiac microtissue
• Engineered heart tissue
• Heart-on-chip
• Cardiomyocyte maturation
• Multicellular cell diseases and drug efficacy platform
• Structural readout
• Functional readout

• All authors: Arslan, U.; Moruzzi, A.; Nowacka, J.; Mummery, C.L.; Eckardt, D.; Loskill, P.; Orlova, V.V.
• Date: 2022-06-01
• Journal: Materials Today Bio
• Volume: 14
• DOI: doi:10.1016/j.mtbio.2022.100259