Monitoring the immune responses to vaccination and pertussis: bordetella pertussis and beyond

Diks, A.M. (2022)

Doctoral Thesis

In this thesis, the monitoring of the immune system in balance and during active responses (by flow cytometry) plays a central role. In chapter 2.1 and 2.2, we investigated the optimal sample logistics for high-dimensional flow cytometry in clinical trials. In chapter 3.1-3.4, we monitored the longitidinal kinetics of circulating immune cells in humans after vaccination or bacterial challenge against Bordetella pertussis. Lastly, in chapter 4, we investigated the immune system in humans carrying a genetic variant of PLCG2 'p.P522R', which is associated with increased longevity and reduced chance of developing dementia.

• Bordetella pertussis
• Tdap
• Human Challenge Model
• Plasma cells
• Vaccination
• Phospholipase C gamma 2
• Alzheimer
• Flow Cytometry
• PLCG2 rs72824905
• clinical trial

• All authors: Diks, A.M.
• Supervisor: Dongen, J.J.M. van
• Co-supervisor: Berkowska, M.A.
• Committee: Orfao, A.; Roestenberg, M.; Bos, N.A.; Holstege, H.
• Qualification: Doctor (dr.)
• Awarding Institution: Faculty of Medicine, Leiden University Medical Center (LUMC), Leiden University
• Date: 2022-12-21