Single-molecule microscopy in zebrafish embryos

Góra, R.J. (2022)

Doctoral Thesis

Single-Molecule Microscopy (SMM) techniques constitute a group of powerful imaging tools that enable researchers to study the dynamic behavior of individual molecules.
In the research described in this doctoral thesis, SMM techniques have been developed to image individual proteins inside cells of a living zebrafish embryo model and to study patterns of their mobility.
The results of the mobility pattern analyses offer new insights into the dynamics of single molecules diffusing inside cells within the context of an intact vertebrate organism.

• Single-molecule microscopy
• Zebrafish
• Protein dynamics
• Mobility patterns
• Diffusion
• Single proteins

• All authors: Góra, R.J.
• Supervisor: Schaaf, M.J.M.; Meijer, A.H.
• Committee: Wezel, G. van; Briegel, A.; Cambi, A.; Loza-Alvarez, P.; Willemse, J.J.
• Qualification: Doctor (dr.)
• Awarding Institution: Institute of Biology Leiden (IBL), Faculty of Science, Leiden University
• Date: 2022-11-23
• ISBN (print): 9789464239065
• Sponsorship: This publication was funded by the Marie Sklodowska-Curie Interactive Training Network (ITN) ImageInLife (Grant Agreement 721537)