Multilayer porous scaffolds for cartilage tissue engineering

Campos Mora, Y. (2022)

Doctoral Thesis

Due to the steep rise of the prevalence of osteoarthritis in the world's population in recent years, it has been of great interest to search for the best way to relieve symptoms and consequences of OA like pain and inflammation. For that reason, the tissue engineering using a porous tri-layer scaffold is a great alternative for patients with this illness.
In this way, it was designed, developed and improved a novel chitosan/collagen-based tri-layer porous scaffold with similar chemical composition and structure to the articular cartilage. One of these layers included hydroxyapatite, to promote the integration of growing tissue next to subchondral bone.
The biomaterial was physic-chemical characterized, in vitro and in vivo tested obtaining promising results. Later, the scaffold was crosslinked to promote rheological properties that bear the knee cyclic charges while walking. The anti-inflammatory effect was enhanced by the incorporation of a TNF-α blocking drug, which was released in a controlled manner from the material in the damaged tissue over time. The scaffold was non-cytotoxic against chondrocytes and osteoblasts, had great rheological properties and promoted the growth of new cartilage tissue after four weeks after grafted into the osteoarthritic mice knee.

• Articular cartilage
• Tissue engineering
• Multilayer scaffolds

• All authors: Campos Mora, Y.
• Supervisor: Bloem, J.L.
• Co-supervisor: Cruz ricondo, L.J.; Fuentes Estévez, G.
• Committee: Meulenbelt, I.; Lelieveldt, B.P.F.; Kaijzel, E.L.; Santos garcía, M.; González de Torre, I.
• Qualification: Doctor (dr.)
• Awarding Institution: Faculty of Medicine, Leiden University Medical Center (LUMC), Leiden University
• Date: 2022-09-07
• ISBN (print): 9789464195385
• Sponsorship: Chipsoft BV, Percuros BV, Leiden University Library