Structure and function of the endothelial glycocalyx in the microcirculation

Dane, M.J.C. (2015)

Doctoral Thesis

The endothelial glycocalyx is a thin layer consisting of sugar moieties on the endothelium of the whole vasculature. This layer has been shown to play a role in inflammation coagulation and permeability in the vasculature. In this thesis we studied the structure and composition of the endothelial glycocalyx. We developed a model to induce an anti-inflammatory endothelial glycocalyx in vitro, using physiolgoical shear stress. Furthermore, we studied the role of this glycocalyx in the glomerular capillaries. Here we show that the endothelial glycocalyx contributes to the filtration barrier in the glomeruli. Consequently this implies that damage of this glycocalyx might be a first step in the development of vasculature-born renal failure, like in Type 2 diabetes. The other way around, we also studied the effect of renal failure on the systemic glycocalyx. Using a non-invasive method, we estimated glycocalyx thickness in the sublingual vasculature of patients with end stage renal disease and patients who received a kidney transplant. Interestingly, the endothelial glycocalyx was highly perturbed during end stage renal disease, but comparable to healthy controls after a kindye transplantation. Altogether the endothelial glycocalyx might be a central mediator in the development of both renal and cardiovascular disease.

• Microvasculature
• SDF
• Shear stress
• Renal
• Kidney
• Glycocalyx
• Endothelium

• All authors: Dane, M.J.C.
• Supervisor: Rabelink, A.J.
• Co-supervisor: Berg, B.M. van den; Vink, H.
• Qualification: Doctor (dr.)
• Awarding Institution: Faculty of Medicine (LUMC), Leiden University
• Date: 2015-06-02
• Publisher: s'-Hertogenbosch: Uitgeverij BOXpress
• ISBN (print): 9789462951631