In Collections
This item can be found in the following collections:
![Microanatomy of advanced human atherosclerotic plaques through single-cell transcriptomics](https://scholarlypublications.universiteitleiden.nl/sites/all/themes/scholarly/img/closed_access.png)
Microanatomy of advanced human atherosclerotic plaques through single-cell transcriptomics
Atherosclerotic lesions are known for their complex and diverse cellular heterogeneity, yet the exact cellular composition of human plaques remains unclear. Here we aim to provide a comprehensive overview of the cellular content of advanced atherosclerotic lesions using single-cell RNA sequencing (scRNAseq), to increase our understanding of the pathophysiologic processes underlying atherosclerosis.
Advanced carotid plaques were obtained from 3 male endarterectomy patients. Plaques were enzymatically digested and live cells were sorted using FACS. Subsequently, we performed scRNAseq on a total of 3456 plaque derived cells.
Unsupervised clustering revealed 11 distinct cell populations, including macrophages, CD4+and CD8+T-cells, B-cells, mast cells, endothelial cells, and smooth muscle cells. Interestingly, we were able to distinguish multiple subclusters for the smooth muscle cells, macrophages, and T-cells. We observed a contractile and synthetic smooth muscle cell...
Show moreAtherosclerotic lesions are known for their complex and diverse cellular heterogeneity, yet the exact cellular composition of human plaques remains unclear. Here we aim to provide a comprehensive overview of the cellular content of advanced atherosclerotic lesions using single-cell RNA sequencing (scRNAseq), to increase our understanding of the pathophysiologic processes underlying atherosclerosis.
Advanced carotid plaques were obtained from 3 male endarterectomy patients. Plaques were enzymatically digested and live cells were sorted using FACS. Subsequently, we performed scRNAseq on a total of 3456 plaque derived cells.
Unsupervised clustering revealed 11 distinct cell populations, including macrophages, CD4+and CD8+T-cells, B-cells, mast cells, endothelial cells, and smooth muscle cells. Interestingly, we were able to distinguish multiple subclusters for the smooth muscle cells, macrophages, and T-cells. We observed a contractile and synthetic smooth muscle cell cluster and three different macrophage clusters, including inflammatory, wound healing and antigen presenting macrophages. Finally, CD8+T-cells could be subdivided into three clusters based on their activation state, with a clear division between cytolytic effectors and quiescent memory CD8+T-cells.
We developed a technique to perform scRNAseq on advanced human atherosclerotic lesions to unravel the cellular landscape within the plaques. We now provide, for the first time, an explicit expression profile of the different cells and their subtypes in atherosclerosis.
Show less- All authors
- Depuydt, M.; Prange, K.H.M.; Slenders, L.; Elbersen, D.; Boltjes, A.; De Jager, S.C.A.; Slutter, B.A.; Bot, I.; Laan, S.W. van der; Mokry, M.; Pasterkamp, G.; Winther, M.P.J. de; Kuiper, J.
- Date
- 2019-08-31
- Volume
- 287
- Pages
- E5 - E5