Leiden University Scholarly Publications

Persistent URL of this record https://hdl.handle.net/1887/3185222

In Collections

This item can be found in the following collections:

Rokidi, S.; Bravenboer, N.; Gamsjaeger, S.; Chavassieux, P.; Zwerina, J.; Paschalis, E.; ... ; Appelman-Dijkstra, N. (2020)

Impact microindentation measurements correlate with cortical bone material properties measured by Fourier transform infrared imaging in humans

Article / Letter to editor
Bone Material Strength index (BMSi) measured by Impact Microindentation is generally lower in subjects with fragility fractures independently of BMD values. We recently reported that in humans, BMSi values are strongly associated with material properties of subperiosteal mineralized bone surface (local mineral content, nanoporosity, pyridinoline content). In the present study we investigated the relationship of BMSi with material properties of the whole bone cortex, by analyzing thin sections of iliac crest biopsies (N = 12) from patients with different skeletal disorders and a wide range of BMD with or without fractures, by Fourier transform infrared imaging (FTIRI). The calculated parameters were: i) mineral and organic matrix content and their ratio (MM), ii) mineral maturity/crystallinity (MMC) and iii) the ratio of pyridinoline (Pyd) and divalent collagen cross-links (XLR). Results were expressed as images, which were converted to histogram distributions. For each histogram the...Show moreBone Material Strength index (BMSi) measured by Impact Microindentation is generally lower in subjects with fragility fractures independently of BMD values. We recently reported that in humans, BMSi values are strongly associated with material properties of subperiosteal mineralized bone surface (local mineral content, nanoporosity, pyridinoline content). In the present study we investigated the relationship of BMSi with material properties of the whole bone cortex, by analyzing thin sections of iliac crest biopsies (N = 12) from patients with different skeletal disorders and a wide range of BMD with or without fractures, by Fourier transform infrared imaging (FTIRI). The calculated parameters were: i) mineral and organic matrix content and their ratio (MM), ii) mineral maturity/crystallinity (MMC) and iii) the ratio of pyridinoline (Pyd) and divalent collagen cross-links (XLR). Results were expressed as images, which were converted to histogram distributions. For each histogram the characteristics recorded were: mean value, mode (most often occurring value), skewness, and kurtosis and their association with BMSi values was examined by correlation analysis. BMSi values were significantly correlated only with MM mean and mode values (r = 0.736, p = 0.0063, and r = 0.855, p = 0.0004, respectively), and with XLR mode values (r = - 0.632, p = 0.0274). The results of the present study demonstrate that BMSi values are strongly associated with MM, a metric that corrects the mineral content for the organic matrix content, and may also depend on organic matrix quality. These and our previous observations strongly suggest that BMSi assesses material properties of cortical bone.Show less
Impact micro indentation
Bone mineral strength index
Material properties
Mineral to matrix content
Bone matrix
FTIR imaging analysis
Iliac crest biopsies
Fractures
All authors
Rokidi, S.; Bravenboer, N.; Gamsjaeger, S.; Chavassieux, P.; Zwerina, J.; Paschalis, E.; Papapoulos, S.; Appelman-Dijkstra, N.
Date
2020-08-01
Journal
BONE
Volume
137
DOI
doi:10.1016/j.bone.2020.115437