RESIST-5 OOKNV and NG-Test Carba 5 assays for the rapid detection of carbapenemase-producing Enterobacterales from positive blood cultures: a comparative study

Bianco, G.; Boattini, M.; Asten, S.A.V. van; Iannaccone, M.; Zanotto, E.; Zaccaria, T.; ... ; Costa, C. (2020)

Article / Letter to editor

We prospectively compared the performance of RESIST-5 O.O.K.N.V. and NG-Test Carba 5 assays directly from blood cultures spiked with 130 characterized Enterobacterales isolates. Overall, both assays yielded 100% sensitivity to detect KPC-type carbapenemases and OXA-48-like carbapenemases. Both assays failed to detect KPC-31 and KPC-33, D179Y point mutation variants of KPC-3 and KPC-2, that are deprived of carbapenemase activity and confer resistance to ceftazidime-avibactam. On blood culture bacterial pellets, NDMand VIM-type carbapenemases were detected in 50.0% and 52.2%, respectively, by RESIST5 O.O.K.N.V. vs 100% by NG-Test Carba 5. The sensitivity of RESIST-5 O.O.K.N.V. improved to 100% and 95.6%, respectively, by performing the assay on 4-h early subculture. (C) 2020 Published by Elsevier Ltd on behalf of The Healthcare Infection Society.

• Carbapenemase detection
• Enterobacterales
• Blood culture
• Immunochromatographic assay
• Ceftazidime-avibactam resistance
• D179Y

• All authors: Bianco, G.; Boattini, M.; Asten, S.A.V. van; Iannaccone, M.; Zanotto, E.; Zaccaria, T.; Bernards, A.T.; Cavallo, R.; Costa, C.
• Date: 2020-06-01
• Journal: Journal of Hospital Infection
• Volume: 105
• Issue: 2
• Pages: 162 - 166
• DOI: doi:10.1016/j.jhin.2020.03.022