Leiden University Scholarly Publications

Persistent URL of this record https://hdl.handle.net/1887/17923

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Sharif, F. (2011)

The zebrafish as a model for tissue regeneration and bone remodelling

Doctoral Thesis
The aim of this thesis was to investigate the expression, and function of genes associated with remodelling and regeneration in the zebrafish model species. Here, we studied the role of cell populations, defined by their expression of markers, in bone regeneration and remodelling in zebrafish embryos and adult zebrafish scales. We used mesoporous silica nanoparticles to carry cytokines, known to activate hematopoietic cells into osteoclasts, into tissues of the living embryo. We further investigated the role of genes such as matrix metalloproteinases in regeneration of adult zebrafish scales. These genes are known to be involved in matrix degradation and have been found to be expressed in mammalian osteoclasts. We studied both by in situ hybridisation and immunocytochemistry the presence of mononucleated and multinucleated mmp-9 positive cells on the episquamal side of adult zebrafish scales. Finally we studied the regeneration of the caudal fin of zebrafish embryos with a...Show moreThe aim of this thesis was to investigate the expression, and function of genes associated with remodelling and regeneration in the zebrafish model species. Here, we studied the role of cell populations, defined by their expression of markers, in bone regeneration and remodelling in zebrafish embryos and adult zebrafish scales. We used mesoporous silica nanoparticles to carry cytokines, known to activate hematopoietic cells into osteoclasts, into tissues of the living embryo. We further investigated the role of genes such as matrix metalloproteinases in regeneration of adult zebrafish scales. These genes are known to be involved in matrix degradation and have been found to be expressed in mammalian osteoclasts. We studied both by in situ hybridisation and immunocytochemistry the presence of mononucleated and multinucleated mmp-9 positive cells on the episquamal side of adult zebrafish scales. Finally we studied the regeneration of the caudal fin of zebrafish embryos with a special emphasis on the effect of glucocorticoids on regeneration and wound healing. Glucocorticoids in this case mimic stress conditions in the embryos, thus helping understand the effect of early exposure to stress on wound healing and tissue Remodelling.Show less
Zebrafish scales
Zebrafish
Regeneration
Osteoclasts
Neutrophils
Mesoporous silica nanoparticles.
Macrophages
Embryos
Bone remodelling
All authors
Sharif, F.
Supervisor
Richardson, M.K.
Qualification
Doctor (dr.)
Awarding Institution
Institute of Biology Leiden (IBL), Faculty of Science, Leiden University
Date
2011-10-12

Juridical information

Court
LEI Universiteit Leiden

Funding

Sponsorship
Higher Education Commission of Pakistan and the Smart Mix Programme of the Netherlands Ministry of Economic Affairs and The Netherlands Ministry of Education, Culture and Science.