High-throughput quantification and unambiguous identification for metabolomics

Laan, T. van der (2020)

Doctoral Thesis

The challenge of achieving fast quantification in metabolomics is the presence of severe matrix effects during the MS analysis of complex samples. Complex samples also result in challenges during metabolite identification as complex MS/MS spectra and peak overlap in 1H NMR complicate structure elucidation.  The goal of this thesis is to tackle these challenges by the development and application of innovative fractionation approaches and state-of-the-art MS and NMR analyses.

• Metabolomics
• Mass spectrometry
• Nuclear magnetic resonance spectroscopy
• Fractionation
• Quantification
• Identification
• Data independent acquisition
• Matrix effect
• Chromatography
• High-throughput

• All authors: Laan, T. van der
• Supervisor: Hankemeier, T.
• Co-supervisor: Dubbelman, A.C.; Harms, A.C.
• Committee: Irth, H.; Bouwstra, J.A.; Schoenmakers, P.J.; Duynhoven, J.P.M. van; Hopfgartner, G.; Lange, E.C.M. de
• Qualification: Doctor (dr.)
• Awarding Institution: Leiden Academic Centre for Drug Research (LACDR), Faculty of Science, Leiden University
• Date: 2020-12-14
• ISBN (print): 9789083111728
• Sponsorship: COAST/NWO