Exploring Grainyhead-like 2 target genes in breast cancer

Wang, Z. (2020)

Doctoral Thesis

The objective of this study was to investigate the expression and function of GRHL2 in different breast cancer subtypes. In Chapter 2, we focused on the expression of GRHL2 in human breast cancer and the distinct effects of GRHL2 knockout on aspects of growth versus migration in basal A and luminal-like subtypes. In Chapter 3, ChIP-seq was used to explore the genomic landscape of GRHL2 binding sites in basal A and luminal-like subtypes of breast cancer and this data was used to predict shared and distinct GRHL2 target genes. In Chapter 4, based on a conditional GRHL2 knockout system, we determined the dynamic changes in genome-wide DNA transcription triggered by loss of GRHL2 in luminal-like breast cancer cells and used the data to predict affected pathways. In Chapter 5, ChIP-seq and BrU-seq data were integrated to identify genes whose transcription is controlled by GRHL2 and establish gene expression networks regulated by GRHL2 in luminal-like breast cancer.

• GRHL2
• ChIP-seq
• Bru-seq
• Breast cancer

• All authors: Wang, Z.
• Supervisor: Danen, E.H.J.
• Co-supervisor: Zweemer, J.M.
• Committee: Irth, H.; Bouwstra, J.A.; Water, B. van de; Martens, J.; Pluijm, G. van der
• Qualification: Doctor (dr.)
• Awarding Institution: Leiden Academic Centre for Drug Research (LACDR), Faculty of Science, Leiden University
• Date: 2020-10-06
• ISBN (print): 9789492597458
• Sponsorship: China Scholarship Council (201506200095), Dutch Cancer Society (grant no. 10967)