Chimerism in health, transplantation and autoimmunity

Koopmans, M.; Kremer Hovinga, I.C.L. (2009)

Doctoral Thesis

The term __chimerism__ originates from Greek mythology and refers to the creature Chimaera, whose body was in front a lion, the back a serpent and the midsection a goat. In medicine, the term chimerism refers to an individual, organ or part consisting of tissues of diverse genetic constitution. Pregnancy, blood transfusion and organ transplantation are potential causes of chimerism. In this thesis the occurrence of chimerism is investigated in different organs of healthy women, of women with the autoimmune disease Systemic Lupus Erythematosus (SLE) and of women that received a renal allograft. To demonstrate chimerism, male cells were detected in female organs by using in situ hybridization of the Y chromosome. Chimerism was found in 18% of healthy organs, in about 50% of organs derived from women with SLE and in none of the skin tumors investigated from female renal allograft recipients. In various organ types and both in women with and without sons and women with and without a transfusion history, chimerism was present. In this thesis we describe these results and review data from the ancient and recent literature. With all these data in hand, we speculate about the sources of chimerism and its implications on immunity.

• Autoimmune disease
• Blood transfusion
• Chimerism
• In situ hybridisation
• Pregnancy
• Systemic Lupus Erythematosus
• Transplantation

• All authors: Koopmans, M.; Kremer Hovinga, I.C.L.
• Supervisor: Brijn, J.A.
• Co-supervisor: Bajema, I.M.
• Qualification: Doctor (dr.)
• Awarding Institution: Leiden University Medical Center (LUMC), Leiden University
• Date: 2009-03-24