Genomic and transcriptomic association studies identify 16 novel susceptibility loci for venous thromboembolism

Lindstrom, S.; Wang, L.; Smith, E.N.; Gordon, W.; Vlieg, A.V.; Andrade, M. de; ... ; INVENT Consortium (2019)

Article / Letter to editor

Venous thromboembolism (VTE) is a significant contributor to morbidity and mortality. To advance our understanding of the biology contributing to VTE, we conducted a genome-wide association study (GWAS) of VTE and a transcriptome-wide association study (TWAS) based on imputed gene expression from whole blood and liver. Wemeta-analyzedGWAS data from18 studies for 30 234 VTE cases and 172 122 controls and assessed the association between 12 923 718 genetic variants and VTE. We generated variant prediction scores of gene expression from whole blood and liver tissue and assessed them for association with VTE. Mendelian randomization analyses were conducted for traits genetically associated with novel VTE loci. We identified 34 independent genetic signals for VTE risk from GWAS meta-analysis, of which 14 are newly reported associations. This included 11 newly associated genetic loci (C1orf198, PLEK, OSMR-AS1, NUGGC/SCARA5, GRK5, MPHOSPH9, ARID4A, PLCG2, SMG6, EIF5A, and STX10) of which 6 replicated, and 3 new independent signals in 3 known genes. Further, TWAS identified 5 additional genetic loci with imputed gene expression levels differing between cases and controls in whole blood (SH2B3, SPSB1, RP11-747H7.3, RP4-737E23.2) and in liver (ERAP1). At some GWAS loci, we found suggestive evidence that the VTE association signal for novel and previously known regions colocalized with expression quantitative trait locus signals. Mendelian randomization analyses suggested that blood traits may contribute to the underlying risk of VTE. To conclude, we identified 16 novel susceptibility loci for VTE; for some loci, the association signals are likely mediated through gene expression of nearby genes.

• All authors: Lindstrom, S.; Wang, L.; Smith, E.N.; Gordon, W.; Vlieg, A.V.; Andrade, M. de; Brody, J.A.; Pattee, J.W.; Haessler, J.; Brumpton, B.; Chasman, D.I.; Suchon, P.; Chen, M.H.; Turman, C.; Germain, M.; Wiggins, K.L.; MacDonald, J.; Braekkan, S.K.; Armasu, S.M.; Pankratz, N.; Jackson, R.D.; Nielsen, J.B.; Giulianini, F.; Puurunen, M.K.; Ibrahim, M.; Heckbert, S.R.; Damrauer, S.M.; Natarajan, P.; Klarin, D.; Vries, P.S. de; Sabater-Lleal, M.; Huffman, J.E.; Bammler, T.K.; Frazer, K.A.; McCauley, B.M.; Taylor, K.; Pankow, J.S.; Reiner, A.P.; Gabrielsen, M.E.; Deleuze, J.F.; O'Donnell, C.J.; Kim, J.; McKnight, B.; Kraft, P.; Hansen, J.B.; Rosendaal, F.R.; Heit, J.A.; Psaty, B.M.; Tang, W.H.; Kooperberg, C.; Hveem, K.; Ridker, P.M.; Morange, P.E.; Johnson, A.D.; Kabrhel, C.; Tregouet, D.A.; Smith, N.L.; Busenkell, E.; Judy, R.; Lynch, J.; Levin, M.; Aragam, J.H.K.; Chaffin, M.; Haas, M.; Assimes, T.L.; Huang, J.; Lee, K.M.; Shao, Q.; Huang, Y.F.; Sun, Y.V.; Vujkovic, M.; Saleheen, D.; Miller, D.R.; Reaven, P.; DuVall, S.; Boden, W.; Pyarajan, S.; Henke, P.; Gaziano, J.M.; Concato, J.; Rader, D.J.; Cho, K.; Chang, K.M.; Wilson, P.W.F.; Tsao, P.S.; Kathiresan, S.; Obi, A.; Million Veteran Program; CHARGE Hemostasis Working Grp; INVENT Consortium
• Date: 2019-11-07
• Journal: Blood
• Volume: 134
• Issue: 19
• Pages: 1645 - 1657
• DOI: doi:10.1182/blood.2019000435