Generation of 5 induced pluripotent stem cell lines, LUMCi007-A and B and LUMCi008-A, B and C, from 2 patients with Huntington disease

Graaf, L.M. van der; Gardiner, S.L.; Tok, M.; Brands, T.; Boogaard, M.W.; Pepers, B.A.; ... ; Roon-Mom, W.M.C. van (2019)

Article / Letter to editor

Huntington disease (HD) is an autosomal dominant, neurodegenerative disease caused by a CAG repeat expansion within the coding sequence of the HTT gene, resulting in a highly toxic protein with an expanded polyglutamine stretch that forms typical protein aggregates throughout the brain. We generated human induced pluripotent stem cells (hiPSCs) from two HD patients using non-integrating Sendai virus (SeV). The hiPSCs display a normal karyotype, express all pluripotency markers, have the same CAG repeat expansion as the original fibroblasts and are able to differentiate into the three germ layers in vitro.

• All authors: Graaf, L.M. van der; Gardiner, S.L.; Tok, M.; Brands, T.; Boogaard, M.W.; Pepers, B.A.; Eussen, B.; Klein, A. de; Aziz, N.A.; Freund, C.; Buijsen, R.A.M.; Roon-Mom, W.M.C. van
• Date: 2019-08-01
• Journal: Stem Cell Research
• Volume: 39
• DOI: doi:10.1016/j.scr.2019.101498