A Randomized Controlled Clinical Trial Comparing Belatacept With Tacrolimus After De Novo Kidney Transplantation

Graav, G.N. de; Baan, C.C.; Clahsen-van Groningen, M.C.; Kraaijeveld, R.; Dieterich, M.; Verschoor, W.; Thusen, J.H. von der; Roelen, D.L.; Cadogan, M.; Wetering, J. van de; Rosmalen, J. van; Weimar, W.; Hesselink, D.A.

doi:doi:10.1097/TP.0000000000001755

Background. Belatacept, an inhibitor of the CD28-CD80/86 costimulatory pathway, allows for calcineurin-inhibitor free immunosuppressive therapy in kidney transplantation but is associated with a higher acute rejection risk than ciclosporin. Thus far, no biomarker for belatacept-resistant rejection has been validated. In this randomized-controlled trial, acute rejection rate was compared between belatacept-and tacrolimus-treated patients and immunological biomarkers for acute rejection were investigated. Methods. Forty kidney transplant recipients were 1:1 randomized to belatacept or tacrolimus combined with basiliximab, mycophenolate mofetil, and prednisolone. The 1-year incidence of biopsy-proven acute rejection was monitored. Potential biomarkers, namely, CD8(+) CD28(-), CD4(+) CD57(+) PD1(-), and CD8(+) CD28(++) end-stage terminally differentiated memory T cells were measured pretransplantation and posttranspla(n)tation and correlated to rejection. Pharmacodynamic monitoring of...Show moreBackground. Belatacept, an inhibitor of the CD28-CD80/86 costimulatory pathway, allows for calcineurin-inhibitor free immunosuppressive therapy in kidney transplantation but is associated with a higher acute rejection risk than ciclosporin. Thus far, no biomarker for belatacept-resistant rejection has been validated. In this randomized-controlled trial, acute rejection rate was compared between belatacept-and tacrolimus-treated patients and immunological biomarkers for acute rejection were investigated. Methods. Forty kidney transplant recipients were 1:1 randomized to belatacept or tacrolimus combined with basiliximab, mycophenolate mofetil, and prednisolone. The 1-year incidence of biopsy-proven acute rejection was monitored. Potential biomarkers, namely, CD8(+) CD28(-), CD4(+) CD57(+) PD1(-), and CD8(+) CD28(++) end-stage terminally differentiated memory T cells were measured pretransplantation and posttranspla(n)tation and correlated to rejection. Pharmacodynamic monitoring of belatacept was performed by measuring free CD86 on monocytes. Results. The rejection incidence was higher in belatacept-treated than tacrolimus-treated patients: 55% versus 10% (P = 0.006). All 3 graft losses, due to rejection, occurred in the belatacept group. Although 4 of 5 belatacept-treated patients with greater than 35 cells CD8(+) CD28(++) end-stage terminally differentiated memory T cells/mu L rejected, median pretransplant values of the biomarkers did not differ between belatacept-treated rejectors and nonrejectors. In univariable Cox regressions, the studied cell subsets were not associated with rejection-risk. CD86 molecules on circulating monocytes in belatacept-treated patients were saturated at all timepoints. Conclusions. Belatacept-based immunosuppressive therapy resulted in higher and more severe acute rejection compared with tacrolimus-based therapy. This trial did not identify cellular biomarkers predictive of rejection. In addition, the CD28-CD80/86 costimulatory pathway appeared to be sufficiently blocked by belatacept and did not predict rejection.Show less

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A Randomized Controlled Clinical Trial Comparing Belatacept With Tacrolimus After De Novo Kidney Transplantation