Generation of 3 human induced pluripotent stem cell lines LUMCi005-A, B and C from a Hereditary Cerebral Hemorrhage with Amyloidosis-Dutch type patient

Daoutsali, E.; Buijsen, R.A.M.; Pas, S. van de; Jong, A. 't; Mikkers, H.; Brands, T.; ... ; Roon-Mom, W.M.C. van (2019)

Article / Letter to editor

Hereditary Cerebral Hemorrhage with Amyloidosis-Dutch type (HCHWA-D) is an autosomal dominant hereditary disease caused by a point mutation in exon 17 of the APP gene. We generated human induced pluripotent stem cells (hiPSCs) from a symptomatic HCHWA-D patient by using non-integrating Sendai virus (SeV). The newly generated hiPSCs express all pluripotency markers, have a normal karyotype, carry the Dutch mutation, can differentiate in the three germ layers in vitro and are SeV free.

• All authors: Daoutsali, E.; Buijsen, R.A.M.; Pas, S. van de; Jong, A. 't; Mikkers, H.; Brands, T.; Eussen, B.; Klein, A. de; Graaf, L.M. van der; Pepers, B.A.; Freund, C.; Terwindt, G.M.; Orlova, V.V.; Roon-Mom, W.M.C. van
• Date: 2019-01-01
• Journal: Stem Cell Research
• Volume: 34
• DOI: doi:10.1016/j.scr.2018.101359