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Eikmans, M.; Waanders, M.M.; Roelen, D.L.; Miert, P.P.M.C. van; Anholts, J.D.H.; Fijter, H.W. de; ... ; Claas, F.H.J. (2010)

Differential Effect of Pretransplant Blood Transfusions on Immune Effector and Regulatory Compartments in HLA-Sensitized and Nonsensitized Recipients

Article / Letter to editor
Background. Blood transfusion (BT) may elicit both harmful and beneficial immune responses against a subsequent organ graft. Immune parameters of a single, nonleukocyte-depleted BT were investigated in two groups: non-human leukocyte antigen (HLA)-sensitized recipients with a one-HLA-DR matched donor (protocolled BT [PBT]) and females with previous exposure to HLA alloantigens through pregnancy (donor-specific transfusion [DST]). Methods. Thirty-five percent of DST recipients and 9.5% of PBT recipients developed HLA antibodies after BT. Phenotypic and functional analyses were performed in pre-BT, 2 weeks post-BT, and more than 10 weeks post-BT samples (PBT: n=10; DST: n=14). Result. The number of donor-reactive interferon-gamma-producing memory T cells increased 2 weeks post-BT, but only in the DST group, increased frequencies persisted beyond 10 weeks (P<0.004). In the DST recipients, the proportion of natural killer cells (CD3(-)CD56(+)) significantly increased after BT (P=0.01),...Show moreBackground. Blood transfusion (BT) may elicit both harmful and beneficial immune responses against a subsequent organ graft. Immune parameters of a single, nonleukocyte-depleted BT were investigated in two groups: non-human leukocyte antigen (HLA)-sensitized recipients with a one-HLA-DR matched donor (protocolled BT [PBT]) and females with previous exposure to HLA alloantigens through pregnancy (donor-specific transfusion [DST]). Methods. Thirty-five percent of DST recipients and 9.5% of PBT recipients developed HLA antibodies after BT. Phenotypic and functional analyses were performed in pre-BT, 2 weeks post-BT, and more than 10 weeks post-BT samples (PBT: n=10; DST: n=14). Result. The number of donor-reactive interferon-gamma-producing memory T cells increased 2 weeks post-BT, but only in the DST group, increased frequencies persisted beyond 10 weeks (P<0.004). In the DST recipients, the proportion of natural killer cells (CD3(-)CD56(+)) significantly increased after BT (P=0.01), whereas in PBT recipients, the proportion of regulatory T cells (CD4(+)CD25(+)Foxp3(+)CD127(low)) significantly increased at 2 weeks post-BT (P=0.039). Microarray analysis confirmed increased activity of genes involved in function of natural killer cells, T cells, and B cells in DST recipients and increased expression of immuneregulatory genes (galectin-1, Foxo3a, and follistatin-like 3) in PBT recipients. Galectin-1 expression by quantitative polymerase chain reaction was significantly enhanced in peripheral blood cells after PBT (P<0.05). Conclusion. Decreased immune effector mechanisms combined with an increased immune regulatory cell signature after HLA-DR-matched BT in nonsensitized patients is in line with clinical observations of improved outcome of a subsequent graft. Previous sensitization, however, may lead to HLA antibody formation and prolonged donor-specific memory T-cell reactivity after BT.Show less
Pretransplant Blood transfusion Cellular immunity Immune regulation HLA t-cells tolerance transplantation galectin-1 rejection immunomodulation inflammation quiescence molecules mechanism
All authors
Eikmans, M.; Waanders, M.M.; Roelen, D.L.; Miert, P.P.M.C. van; Anholts, J.D.H.; Fijter, H.W. de; Brand, A.; Claas, F.H.J.
Date
2010-12-15
Journal
Transplantation
Volume
90
Issue
11
Pages
1192 - 1199
DOI
doi:10.1097/TP.0b013e3181fa943d