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Robles, A.; Medeiros, A.; Berois, N.; Balter, H.S.; Pauwels, E.K.; Osinaga, E. (2010)

In-site interaction evaluation of Tn density by inhibition/competition assays

Article / Letter to editor
The tumor-associated structure N-acetyl-galactosamine-O-Ser/Thr (Tn antigen), which is overexpressed in various tumor cell types, notably of the breast, ovary and colon, is an interesting determinant that is useful for cancer diagnosis and follow-up. The aim of this research was to study different assay strategies in order to determine the most sensitive system for further application in epitope characterization and binding assessment. The tetrameric isolectin obtained from Vicia villosa seeds (VVLB4) shows high affinity for the tumor-associated structure. A monoclonal antibody against VVLB4, MabVV(34), was generated, and the interaction between MabVV(34) and VVLB4 was studied by means of binding and inhibition assays. Several synthetic peptides (10 amino acid sequences) designed from the amino acid sequence of VVLB4 and obtained from trypsin digestion were tested to determine which amino acids were involved in the interaction between MabVV(34) and VVLB4. The further unraveling of...Show moreThe tumor-associated structure N-acetyl-galactosamine-O-Ser/Thr (Tn antigen), which is overexpressed in various tumor cell types, notably of the breast, ovary and colon, is an interesting determinant that is useful for cancer diagnosis and follow-up. The aim of this research was to study different assay strategies in order to determine the most sensitive system for further application in epitope characterization and binding assessment. The tetrameric isolectin obtained from Vicia villosa seeds (VVLB4) shows high affinity for the tumor-associated structure. A monoclonal antibody against VVLB4, MabVV(34), was generated, and the interaction between MabVV(34) and VVLB4 was studied by means of binding and inhibition assays. Several synthetic peptides (10 amino acid sequences) designed from the amino acid sequence of VVLB4 and obtained from trypsin digestion were tested to determine which amino acids were involved in the interaction between MabVV(34) and VVLB4. The further unraveling of this epitope was investigated by inhibition using designed synthetic peptides as well as mixtures mimicking variable density effect. Under the experimental circumstances, MabVV(34) was able to inhibit the binding of VVLB4 to Tn. Two of the four peptide sequences assayed showed better inhibition properties. Finally, mixtures containing these selected sequences allowed the evaluation of binding and inhibition as a function of Tn density. We conclude that the present study facilitates the further development of a specific Tn marker and may contribute to the development of Tn-like radiolabelled peptides or Tn-specific radiolabelled fragments providing a highly selective tool for cancer diagnosis and treatment. This strategy may contribute to characterize the new generation of radiopharmaceuticals for diagnosis and therapy based on biomolecules like antibodies, fragments or peptides, whose application is directly guided by their specific molecular recognition. (C) 2010 Published by Elsevier Inc.Show less
In-site interaction Tn density Inhibition/competition assays vicia-villosa seeds monoclonal-antibody breast-cancer isolectin b4 antigen lectin glycodynamics specificity expression prognosis
All authors
Robles, A.; Medeiros, A.; Berois, N.; Balter, H.S.; Pauwels, E.K.; Osinaga, E.
Date
2010-05-31
Journal
Nuclear Medicine and Biology
Volume
37
Issue
4
Pages
453 - 458
DOI
doi:10.1016/j.nucmedbio.2009.10.009