Leiden University Scholarly Publications

Persistent URL of this record https://hdl.handle.net/1887/110442

In Collections

This item can be found in the following collections:

Schenk, G.J.; Veldhuisen, B.; Wedemeier, O.; McGown, C.C.; Schouten, T.G.; Oitzl, M.; ... ; Vreugdenhil, E. (2010)

Over-expression of delta C-DCLK-short in mouse brain results in a more anxious behavioral phenotype

Article / Letter to editor
Products of the Doublecortin-Like Kinase (DCLK) gene are associated with cortical migration and hippocampal maturation during embryogenesis However the functions of those DCLK gene transcripts that encode kinases and are expressed during adulthood are Incompletely understood To elucidate potential functions of these DCLK gene splice variants we have generated and analyzed transgenic mice with neuronal over-expression of a truncated constitutively active form of DCLK-short designated delta C-DCLK-short Previously we have performed an extensive molecular characterization of these transgenic delta C-DCLK-short mice and established that a specific subunit of the GABA(A) receptor which is involved in anxiety-related GABAergic neurotransmission is down regulated in the hippocampus Here we show that delta C-DCLK-short mRNA is highly expressed in the hippocampus cortex and amygdala of transgenic mice We provide evidence that the delta C DCLK-short protein is expressed and functional In...Show moreProducts of the Doublecortin-Like Kinase (DCLK) gene are associated with cortical migration and hippocampal maturation during embryogenesis However the functions of those DCLK gene transcripts that encode kinases and are expressed during adulthood are Incompletely understood To elucidate potential functions of these DCLK gene splice variants we have generated and analyzed transgenic mice with neuronal over-expression of a truncated constitutively active form of DCLK-short designated delta C-DCLK-short Previously we have performed an extensive molecular characterization of these transgenic delta C-DCLK-short mice and established that a specific subunit of the GABA(A) receptor which is involved in anxiety-related GABAergic neurotransmission is down regulated in the hippocampus Here we show that delta C-DCLK-short mRNA is highly expressed in the hippocampus cortex and amygdala of transgenic mice We provide evidence that the delta C DCLK-short protein is expressed and functional In addition we examined anxiety-related behavior in delta C-DCLK short mice in the elevated plus maze Interestingly delta C-DCLK-short mice spend less time move less in the open arms of the maze and show a reduction in the number of rim dips These behaviors indicate that delta C DCLK-short mice display a more anxious behavioral phenotype (C) 2010 Elsevier Inc All rights reservedShow less
Transgenic mice DCLK DCX In situ hybridization Elevated plus maze GABA Anxiety Hippocampus doublecortin-like-kinase dependent-protein-kinase elevated plus-maze ventral hippocampus neuronal migration amygdaloid-lesions gaba(a) receptors splice variants rat hippocampus deficient mice
All authors
Schenk, G.J.; Veldhuisen, B.; Wedemeier, O.; McGown, C.C.; Schouten, T.G.; Oitzl, M.; Kloet, E.R. de; Vreugdenhil, E.
Date
2010-11-02
Journal
Physiology and Behavior
Volume
101
Issue
4
Pages
541 - 548
DOI
doi:10.1016/j.physbeh.2010.08.002