Leiden University Scholarly Publications

Persistent URL of this record https://hdl.handle.net/1887/110197

In Collections

This item can be found in the following collections:

Hassane, S.; Claij, N.; Jodar, M.; Dedman, A.; Lauritzen, I.; Duprat, F.; ... ; Peters, D.J.M. (2011)

Pkd1-inactivation in vascular smooth muscle cells and adaptation to hypertension

Article / Letter to editor
Autosomal dominant polycystic kidney disease (ADPKD) is a multisystem disorder characterized by renal, hepatic and pancreatic cyst formation and cardiovascular complications. The condition is caused by mutations in the PKD1 or PKD2 gene. In mice with reduced expression of Pkd1, dissecting aneurysms with prominent media thickening have been seen. To study the effect of selective disruption of Pkd1 in vascular smooth muscle cells (SMCs), we have generated mice in which a floxed part of the Pkd1 gene was deleted by Cre under the control of the SM22 promotor (SM22-Pkd1(del/del) mice). Cre activity was confirmed by X-gal staining using lacZ expressing Cre reporter mice (R26R), and quantitative PCR indicated that in the aorta Pkd1 gene expression was strongly reduced, whereas Pkd2 levels remained unaltered. Histopathological analysis revealed cyst formation in pancreas, liver and kidneys as the result of extravascular Cre activity in pancreatic ducts, bile ducts and in the glomerular...Show moreAutosomal dominant polycystic kidney disease (ADPKD) is a multisystem disorder characterized by renal, hepatic and pancreatic cyst formation and cardiovascular complications. The condition is caused by mutations in the PKD1 or PKD2 gene. In mice with reduced expression of Pkd1, dissecting aneurysms with prominent media thickening have been seen. To study the effect of selective disruption of Pkd1 in vascular smooth muscle cells (SMCs), we have generated mice in which a floxed part of the Pkd1 gene was deleted by Cre under the control of the SM22 promotor (SM22-Pkd1(del/del) mice). Cre activity was confirmed by X-gal staining using lacZ expressing Cre reporter mice (R26R), and quantitative PCR indicated that in the aorta Pkd1 gene expression was strongly reduced, whereas Pkd2 levels remained unaltered. Histopathological analysis revealed cyst formation in pancreas, liver and kidneys as the result of extravascular Cre activity in pancreatic ducts, bile ducts and in the glomerular Bowman's capsule. Remarkably, we did not find any spontaneous gross structural blood vessel abnormalities in mice with somatic Pkd1 gene disruption in SMCs or simultaneous disruption of Pkd1 in SMCs and endothelial cells (ECs). Extensive isometric myographic analysis of the aorta did not reveal differences in response to KCl, acetylcholine, phenylephrin or serotonin, except for a significant increase in contractility induced by phenylephrin on arteries from 40 weeks old Pkd1(del/+) germ-line mice. However, SM22-Pkd1(del/del) mice showed significantly reduced decrease in heart rate on angiotensin II-induced hypertension. The present findings further demonstrate in vivo, that adaptation to hypertension is altered in SM22-Pkd1(del/del) mice. Laboratory Investigation (2011) 91, 24-32; doi:10.1038/labinvest.2010.159; published online 20 September 2010Show less
glomerular cysts hypertension Pkd1 polycystic kidney disease SM22Cre Tie2Cre polycystic kidney-disease intracranial aneurysms pkd1 gene expression mice defects tissue inactivation cystogenesis resistance
All authors
Hassane, S.; Claij, N.; Jodar, M.; Dedman, A.; Lauritzen, I.; Duprat, F.; Koenderman, J.S.; Wal, A. van der; Breuning, M.H.; Heer, E. de; Honore, E.; DeRuiter, M.C.; Peters, D.J.M.
Date
2011-01-31
Journal
Laboratory Investigation
Volume
91
Issue
1
Pages
24 - 32
DOI
doi:10.1038/labinvest.2010.159