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Rodriguez, P.G.; Eikenboom, H.C.J.; Tesselaar, M.E.T.; Huisman, M.V.; Nijkeuter, M.; Osanto, S.; Bertina, R.M. (2010)

Plasma levels of microparticle-associated tissue factor activity in patients with clinically suspected pulmonary embolism

Article / Letter to editor
Introduction: Microparticles (MPs) carrying active tissue factor (TF) have been detected in the plasma of cancer patients in particular in those presenting with acute deep vein thrombosis (DVT) or pulmonary embolism (PE). Experimental studies in mice have revealed that circulating MPs carrying TF contribute to thrombus formation. Aim: To study whether unselected patients with an acute confirmed PE have elevated TF activity in the MP fraction (MP-TF activity). Materials and Methods: Plasma MP-TF activity was measured in 159 non-selected patients with clinically suspected PE and in 48 healthy controls as previously described. Blood was collected at time of inclusion. The diagnosis of acute PE was confirmed in 54 patients and excluded in 105 patients. Results: Median MP-TF activity in 159 patients with clinically suspected PE was 72 fM Xa/min [range 32-6657] fM Xa/min and higher than in healthy controls (66 [range 28-183] fM Xa/min; P<0.05). There was no significant difference (P=0.169...Show moreIntroduction: Microparticles (MPs) carrying active tissue factor (TF) have been detected in the plasma of cancer patients in particular in those presenting with acute deep vein thrombosis (DVT) or pulmonary embolism (PE). Experimental studies in mice have revealed that circulating MPs carrying TF contribute to thrombus formation. Aim: To study whether unselected patients with an acute confirmed PE have elevated TF activity in the MP fraction (MP-TF activity). Materials and Methods: Plasma MP-TF activity was measured in 159 non-selected patients with clinically suspected PE and in 48 healthy controls as previously described. Blood was collected at time of inclusion. The diagnosis of acute PE was confirmed in 54 patients and excluded in 105 patients. Results: Median MP-TF activity in 159 patients with clinically suspected PE was 72 fM Xa/min [range 32-6657] fM Xa/min and higher than in healthy controls (66 [range 28-183] fM Xa/min; P<0.05). There was no significant difference (P=0.169) in MP-TF activity between patients with confirmed PE (median 84.5 fM Xa/min [range 36-2149]) and patients without PE (72 fM Xa/min [range 32-6657]) fM Xa/min). In the 159 patients with clinically suspected PE we observed in an exploratory analysis higher MP-TF activity levels in patients with active cancer (median 137 fM Xa/min [range 36-6657]) and cardiovascular disease (median 131.5 fM Xa/min [range 45-2149]) than in patients without these disorders (P=0.0004 and P=0.014, respectively). Conclusion: In patients presenting with clinically suspected PE plasma MP-TF activity was not associated with confirmed PE. (C) 2010 Elsevier B.V. All rights reserved.Show less
Microparticles Tissue factor Thrombosis Pulmonary embolism Cancer venous thrombosis factor expression cancer-patients procoagulant microparticles coagulation activation pancreatic-cancer tumor-cells p-selectin in-vivo thromboembolism
All authors
Rodriguez, P.G.; Eikenboom, H.C.J.; Tesselaar, M.E.T.; Huisman, M.V.; Nijkeuter, M.; Osanto, S.; Bertina, R.M.
Date
2010-10-31
Journal
Thrombosis Research
Volume
126
Issue
4
Pages
345 - 349
DOI
doi:10.1016/j.thromres.2010.07.006