Comparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles-Part 2: Mass spectrometric methods

Reusch, D.; Haberger, M.; Falck, D.; Peter, B.; Maier, B.; Gassner, J.; ... ; Wuhrer, M. (2015)

Article / Letter to editor

• IgG glycosylation
• monoclonal antibody (mAb)
• HILIC-UPLC
• method comparison
• mass spectrometry
• ESI-MS
• MALDI-MS
• LC-MS
• glycan analysis
• PNGase F
• Peptide-N-Glycosidase F
• mAb
• monoclonal antibody
• Fc
• fragment crystallizable
• HILIC-UHPLC
• hydrophilic interaction liquid chromatography-ultra high performance liquid chromatography
• 2-AB
• 2-aminobenzamide
• Fab
• fragment antigen-binding
• IgG
• immunoglobulin G
• PGC-MS
• porous graphitized carbon chromatography- mass spectrometry
• electrospray ionization-mass spectrometry
• MALDI
• matrix assisted laser desorption ionization
• IdeS protease
• proteolytic enzyme like protease from Streptococcus pyrogenes
• HPAEC-PAD
• high-performance anion exchange chromatography with pulsed amperometric detection
• RP-HPLC
• reversed phase high performance liquid chromatography
• CE
• capillary electrophoresis
• TIC
• total ion chromatogram
• LCMS
• liquid chromatography-mass spectrometry

• All authors: Reusch, D.; Haberger, M.; Falck, D.; Peter, B.; Maier, B.; Gassner, J.; Hook, M.; Wagner, K.; Bonnington, L.; Bulau, P.; Wuhrer, M.
• Date: 2015-07-04
• Journal: mAbs
• Volume: 7
• Issue: 4
• Pages: 732 - 742
• DOI: doi:10.1080/19420862.2015.1045173