In the context of cancer, whether or not to choose pregnancy termination represents a difficult and multifaceted decision. In this editorial, members of The Oncologist editorial team attempt to... Show moreIn the context of cancer, whether or not to choose pregnancy termination represents a difficult and multifaceted decision. In this editorial, members of The Oncologist editorial team attempt to contextualize the potential implications of the recent Supreme Court decision in Dobbs v. Jackson Women's Health Organizationfor patients with cancer. Show less
A clinical guideline is a document with the aim of guiding decisions based on evidence regarding diagnosis, management and treatment in specific areas of healthcare. Specific to rheumatic and... Show moreA clinical guideline is a document with the aim of guiding decisions based on evidence regarding diagnosis, management and treatment in specific areas of healthcare. Specific to rheumatic and musculoskeletal diseases (RMDs), adherence to clinical guidelines recommendations impacts the outcomes of people with these diseases. However, currently, the implementation of recommendations is less than optimal in rheumatology.The WHO has described the implementation of evidence-based recommendations as one of the greatest challenges facing the global health community and has identified the importance of scaling up these recommendations. But closing the evidence-to-practice gap is often complex, time-consuming and difficult. In this context, the implementation science offers a framework to overcome this scenario. This article describes the principles of implementation science to facilitate and optimise the implementation of clinical recommendations in RMDs. Embedding implementation science methods and techniques into recommendation development and daily practice can help maximise the likelihood that implementation is successful in improving the quality of healthcare and healthcare services. Show less
The data described was acquired as part of a clinical study with the aim to investigate the potential of tumor-reactive T-cell response as response to vaccination of pancreatic can-cer patients... Show moreThe data described was acquired as part of a clinical study with the aim to investigate the potential of tumor-reactive T-cell response as response to vaccination of pancreatic can-cer patients with an allogenic tumor cell lysate vaccine (Lau et al., 2022). Proteomics analysis was carried out to iden-tify tumor antigens that are shared between the allogeneic tumor cell lysate used for the vaccine and pancreatic duc-tal adenocarcinoma (PDAC) tissue samples. To this objective, cell lysates of the vaccine and of nine tissue samples were enzymatically digested and isotopically labeled with tandem mass tags (TMT) in a so-called six-plex manner (Thermo Fisher Scientific). Three pools were prepared by mixing the samples according to their TMT-labels. Subsequently, the three sample pools were fractionated into 24 fractions with high-pH reversed phase chromatography. These frac-tions were first analyzed on a nano-liquid chromatog-raphy (LC) system online coupled to a high-resolution Eclipse Orbitrap mass spectrometer (MS) equipped with a high-field asymmetric-waveform ion-mobility spectrome-try (FAIMS) source using a data-dependent MS2 shotgun method. Overall, 126,618 unique peptide sequences, on ba-sis of 768,638 peptide spectra matches and corresponding to 7,597 protein groups, were identified in the total sam-ple set including 61 tumor antigens (Supplement Table S2 in Lau et al. 2022) that were prioritized by Cheever and co-workers as vaccine target antigens on basis of a series of objective criteria (Cheever et al., 2009). In the second phase of the experiment, this set of tumor antigens was tar-geted using a serial precursor selection (SPS) MS3 method. From this data, ion trap MS2 and Orbitrap MS3 fragment spectra were extracted for peptide identification (protein se-quence database-dependent search) and relative quantifica-tion using the TMT labels, respectively. The dataset ulti-mately allowed the identification and quantification of 51 proteins and 163 related peptide precursors with the TMT labels (see Fig. 2B and Supplemental Fig. 8, Lau et al. 2022).(c) 2022 Published by Elsevier Inc. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ ) Show less
De afgelopen jaren zijn zowel in het beleid om armoede tegen te gaan als in de maatschappelijke denkbeelden over armoede een aantal zaken ten goede veranderd. Dat betekent niet dat we nu op onze... Show moreDe afgelopen jaren zijn zowel in het beleid om armoede tegen te gaan als in de maatschappelijke denkbeelden over armoede een aantal zaken ten goede veranderd. Dat betekent niet dat we nu op onze han den kunnen gaan zitten. Het aantal huishoudens dat met armoede en schulden kampt, stijgt immers nog steeds. Roeland van Geuns en Mirre Stallen zetten uiteen wat er volgens hen nog meer nodig is om daad werkelijk een eind te maken aan armoede en schuldenproblematiek Show less
Eikmans, M.; Morales-Prieto, D.M.M.; Hoorn, M.L. van der; Markert, U.R.R. 2022
Huntington's disease is the most frequent autosomal dominant neurodegenerative disorder; however, no disease-modifying interventions are available for patients with this disease. The molecular... Show moreHuntington's disease is the most frequent autosomal dominant neurodegenerative disorder; however, no disease-modifying interventions are available for patients with this disease. The molecular pathogenesis of Huntington's disease is complex, with toxicity that arises from full-length expanded huntingtin and N-terminal fragments of huntingtin, which are both prone to misfolding due to proteolysis; aberrant intron-1 splicing of the HTT gene; and somatic expansion of the CAG repeat in the HTT gene. Potential interventions for Huntington's disease include therapies targeting huntingtin DNA and RNA, clearance of huntingtin protein, DNA repair pathways, and other treatment strategies targeting inflammation and cell replacement. The early termination of trials of the antisense oligonucleotide tominersen suggest that it is time to reflect on lessons learned, where the field stands now, and the challenges and opportunities for the future. Show less
Recentelijk verscheen Discours sur le colonialisme van de Martinikaanse denker Aimé Césaire in een mooie Nederlandse vertaling van Grâce Ndjako. Waarom is dit boek zo belangrijk geweest voor de... Show moreRecentelijk verscheen Discours sur le colonialisme van de Martinikaanse denker Aimé Césaire in een mooie Nederlandse vertaling van Grâce Ndjako. Waarom is dit boek zo belangrijk geweest voor de antikoloniale strijd in de 20e eeuw? En wat kan het betekenen voor het Nederlandse debat over het koloniale verleden? Show less
Neuromuscular disorders (NMDs) are a heterogenous group of rare inherited diseases that compromise the function of peripheral nerves and/or muscles. With limited treatment options available, there... Show moreNeuromuscular disorders (NMDs) are a heterogenous group of rare inherited diseases that compromise the function of peripheral nerves and/or muscles. With limited treatment options available, there is a growing need to design effective preclinical studies that can lead to greater success in clinical trials for novel therapeutics. Here, I discuss recent advances in modelling NMDs to improve preclinical studies as well as two articles from this issue that work in parallel to enable a deeper understanding of a particularly rare NMD, known as X-linked myotubular myopathy. Show less
