Comparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles-Part 1: Separation-based methods

Author(s):
Reusch, D.; Haberger, M.; Maier, B.; Maier, M.; Kloseck, R.; Zimmermann, B.; Hook, M.; Szabo, Z.; Tep, S.; Wegstein, J.; Alt, N.; Bulau, P.; Wuhrer, M. (0000-0002-0814-4995)
Published
2015
Publication type
Article / Letter to editor
IgG glycosylationmonoclonal antibody (mAb)HILIC-UPLC2-AB labelingAPTS labelingmethod comparisonDNA analyzerHPAECglycan analysisCE-LIFhigh-throughputmAbmonoclonal antibodyFcfragment crystallizableIgGimmunoglobulin Ghydrophilic interaction liquid chromatography-ultra high performance liquid chromatography2-AB2-aminobenzamideFabfragmentantigen-bindingcapillary electrophoresis-laser induced fluorescenceHPLChigh performance liquid chromatographyMALDI-MSmatrix-assisted laser desorptionionization-mass spectrometryESI-MSelectrospray ionization-mass spectrometryHPAEC-PADhigh-performance anion exchange chromatography with pulsed amperometric detectionAPTS8-aminopyrene-136-trisulfonic acidDSA-FACEDNA-sequencer-aided fluorophore-assisted carbohydrate electrophoresisANTS8-aminonaphthalene-16-trisulfonateCCGEcartridge-based capillary gel electrophoresisHRhigh resolutionIABInstantAB labelingCHOChinese hamster ovary
Date issued
2015-02-28
Language
English
Is part of
mAbs (2015), Vol. 7, no. 1, p. 167-179
hdl
1887/99561
doi
10.4161/19420862.2014.986000