Isolation of the Xenopus homologue of int-1/wingless and expression during neurula stages of early development

Author(s):
Noordermeer, J.N.; Meijlink, F.; Verrijzer, P.; Rijsewijk, F.; Destrée, O.
Published
1989
Publication type
Article / Letter to editor

Abstract

We have isolated the Xenopus homolog (Xint-l) of the mouse protooncogene int-I from a neurula stage 17 cDNA library. The deduced protein sequence of Xint-I includes 371 amino acids. The Xint-l protein is more similar to the mammalian int-I product (69%), than to the Drosophila counterpart of int-1, wingless (50%). Xint-I shares several characteristics of secreted proteins with the other int-I homologs: it has a hydrophobic leader, multiple conserved potential N-linked glycosylation sites and is rich in cysteine residues. All 23 cysteines are conserved in the three proteins. Xint-1 is transiently expressed during the neurula stages of early Xenopus development.

Date issued
1989
Language
English
Is part of
Nucleic Acids Res (1989), Vol. 17, p. 11-18
hdl
1887/839