Towards functional analysis of cerebrovascular cell types derived from human induced pluripotent stem cells

Author(s):
Halaidych, O.V.
Published
2019
Publication type
Doctoral Thesis

Abstract

The work presented in this thesis provides novel contributions to advanced quantitative functional assessment of vascular endothelial and smooth muscle cells differentiated from hiPSCs. The automated assays described provide an unbiased and quantitative assessment of the functionality of hiPSC derivatives which are extremely needed for disease modeling and drug screening. Also, we have demonstrated the consistency of results obtained from cells differentiated from multiple healthy hiPSC lines which makes them good candidates for realistic in vitro models of human vasculature.

Human Induced Pluripotent Stem CellshiPSC-derived endothelial cellshiPSC-derived smooth muscle cellsendothelial cell barrier functionimpedance spectroscopyjunctional integrityinflammatory responsesCa2+ releasecell contractionautomated image analysis
Defense date
2019-11-20
Language
English
Supervisor(s)
Mummery, C.L.
Advisor(s)
Orlova, V.V.
Committee member(s)
Zonneveld, A.J. van; Lebrin, F.P.G.; Maassen van den Brink, A.; Hordijk, P.L.
Qualification
Ph.D.
Degree granting institution
Leiden University of Medical Center [LUMC], Leiden University, Leiden
hdl
1887/80758
ISBN(s)
9786177450152