Biology of cytokine-induced hematopoietic stem and progenitor cell mobilization

Kruijf, E.J.F.M. de (2019)

Doctoral Thesis

Hematopoietic stem and progenitor cells (HSPC) reside in specific niches in the bone marrow, where they are part of a complex micro-environment. Several cytokines, chemokines and small molecules are involved in mobilization of HSPC from the bone marrow towards peripheral blood. This thesis describes several studies on the mechanisms of HSPC mobilization: the kinetics of HSPC mobilization following the administration of Flt3-ligand in a murine model, the effects of repeated in vivo administration of recombinant human G-CSF and murine recombinant G-CSF in mice, the role of alpha-1-antitrypsin in steady state and during G-CSF-induced mobilization in humans and the effects of mesenchymal stromal cells on the stem cell niche in a murine model of G-CSF-induced HSPC mobilization.

• Hematopoietic Stem Cell Mobilization
• Hematopoietic Stem Cell Niche
• Mesenchymal Stromal Cell
• Granulocyte Colony Stimulating Factor
• Alpha-1-antitrypsin

• All authors: Kruijf, E.J.F.M. de
• Supervisor: Fibbe, W.E.
• Co-supervisor: Pel, M. van
• Committee: Zwaginga, J.J.; Cornelissen, J.J.; Schoot, C.E. van der
• Qualification: Doctor (dr.)
• Awarding Institution: Faculty of Medicine, Leiden University Medical Center (LUMC), Leiden University
• Date: 2019-11-21
• ISBN (print): 9789492303264